Stereomicroscope

A stereomicroscope is an optical instrument that produces a three-dimensional view of a specimen, making it valuable for examining biological structures and manipulating samples. It uses two separate optical paths to provide slightly different images to the observer’s eyes, creating depth perception, while reflected or transmitted light illuminates the specimen. With greater working distance and lower magnification than a compound microscope, a stereomicroscope supports the observation and dissection of insects, plants, tissues, embryos, and other relatively large specimens. In biology, it is also useful for sorting samples, performing microdissections, documenting morphology, and guiding procedures that require precise handling.

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Research

JoVE EoE - Neuronal Culture Techniques

Studying the Regeneration of Functional Connections between Spinal Cord Slices Using a Multi-Electrode Array

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2025

The video demonstrates the functional regeneration of propriospinal connections using a multi-electrode array (MEA)-based assay. The spinal cord slices are placed on the MEA and are allowed to fuse. The fused slices are mechanically separated by creating a lesion, inducing propriospinal neurons to grow over the lesion and connect the slices. The electrical activity of the slices is assessed to identify the regeneration of functional connections.

Developing a Micro-Tissue-Engineered Neural Network Using a Hydrogel-Based Micro-column

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2025

Source: Struzyna, L. A. et. al., Anatomically Inspired Three-dimensional Micro-tissue Engineered Neural Networks for Nervous System Reconstruction, Modulation, and Modeling. J. Vis. Exp. (2017)This video demonstrates the development of micro-tissue-engineered neural networks using a hydrogel micro-column with an extracellular matrix core. Seeded neuronal aggregates adhere, extend projections, and form structured neural networks, contributing to advancements in neurodevelopment and...

Establishing an Infection Model Through Microinjection of Gastric Organoids with Pathogenic Bacteria

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2025

This video demonstrates a method to establish a gastric organoid infection model by microinjecting gastric pathogen Helicobacter pylori into the lumen of the organoids. This model helps in studying infection-induced changes in gastric cells.

Intradermal Immunization with Live Attenuated Sporozoites in a Mouse Model

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2025

This video demonstrates an assay for immunizing a mouse model with live attenuated sporozoites. The radiation-attenuated Plasmodium sporozoites are injected into the dorsal side of the mouse's ear. After injections at multiple locations on both ears, the mouse is allowed to recover.

Fluorescence-guided Tumor Resection and MSC-seeded Scaffold Implantation in Murine Model: A Surgical Procedure to Deliver Therapeutic Mesenchymal Stem Cells into Brain Tumor Resection Cavity in Mouse Model

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2025

This video presents the surgical procedure for image-guided tumor resection and implantation of a bio-scaffold seeded with genetically engineered mesenchymal stem cells or MSCs expressing tumor necrosis factor-related apoptosis-inducing ligand or TRAIL. These therapeutic MSCs have the potential in treatment of brain tumors.

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