Hepatocytes
Hepatocytes - Related Videos
07:09
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
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Cited by 93 •
2013
A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.
08:32
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
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Cited by 7 •
2017
Here, we show an enzymatic approach to isolate primary hepatocytes from adult mice, and we describe the quantification of an inflammatory response using ELISA and real-time PCR.
11:24
Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice
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Cited by 1 •
2018
We have described a protocol for performing partial hepatectomy (PHx) and cell transplantation via spleen in NOD.SCID (NOD.CB17-Prkdcscid/J) mice. In this protocol, an incision is made to expose and resect the left lobe of the liver followed by another incision for the intrasplenic transplantation of cells.
08:14
Hepatocyte-specific Ablation in Zebrafish to Study Biliary-driven Liver Regeneration
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Cited by 18 •
2015
To help assess the molecular mechanisms underlying zebrafish biliary-driven liver regeneration, we established a liver injury model in which the nitroreductase-expressing hepatocytes are genetically ablated upon metronidazole treatment. In this protocol, we describe how to adeptly manipulate, monitor and analyze hepatocyte ablation and biliary-driven liver regeneration.
08:36
Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
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Cited by 45 •
2017
The method presented here describes a scalable and good manufacturing practice (GMP)-ready differentiation system to generate human hepatocyte-like cells from pluripotent stem cells. It serves as a cost-effective and standardized system to generate human hepatocyte-like cells for basic and applied human liver research.
FAQs
Their arrangement within liver lobules supports efficient exchange with circulating blood. This positioning allows hepatocytes to access nutrients and foreign compounds delivered through the circulation, process them through biochemical pathways, and release products toward either the bloodstream or bile. Lobular organization therefore connects cellular activity with the liver’s broader transport and processing functions.
Enzyme-driven biochemical reactions allow hepatocytes to transform nutrients and foreign compounds. These reactions support metabolic processing and detoxification by converting incoming substances into forms the cell can handle or route onward. Because hepatocytes use these reactions for both normal metabolism and compound processing, enzyme activity is central to studying metabolic disease and toxic injury.
Hepatocytes direct different products into distinct transport routes: bile or the bloodstream. Release into bile supports the liver’s bile-producing function, whereas secretion into blood contributes to circulating protein and other liver-derived products. Distinguishing these destinations helps researchers interpret how hepatocyte activity contributes to digestion-related liver functions and systemic physiology.
Hepatocytes can store energy and release it when needed, linking cellular metabolism with whole-body nutrient handling. Their ability to process nutrients and adjust whether energy is retained or made available helps explain why hepatocyte function is important in investigations of metabolic disease. This activity also provides a cellular basis for studying changes in nutrient processing.
Research can use primary hepatocytes, cultured hepatocytes, and hepatocyte-like cells. These preparations provide experimental systems for examining liver-specific cellular functions while addressing different research needs. Comparing such models helps investigators study hepatocyte behavior, disease-related changes, and responses to compounds in controlled settings, without limiting liver research to observations of intact tissue.
Cultured hepatocytes provide a liver-relevant system for evaluating how compounds are processed and how they may affect liver-specific cellular functions. In drug screening, this makes them useful for examining responses to candidate substances before broader studies. Their application connects hepatocyte biochemistry, detoxification-related activity, and assessment of potential toxic injury.
Hepatocytes support investigations of liver development, regeneration, metabolic disease, and toxic injury. Researchers can also use hepatocyte-based models for disease modeling and for studying liver-specific cellular function. Together, these applications connect basic cell biology with pathological processes, helping explain how changes in hepatocyte activity relate to the condition and recovery of liver tissue.