Internal Tissue Colonization
Internal Tissue Colonization - Related Videos
06:59
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
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Cited by 43 •
2013
The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.
03:20
Electrophysiological Recording from Colonic Afferent Nerves in an Ex Vivo Rat Colon
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2025
This video demonstrates a method to record the activity of colonic afferent nerves in an ex-vivo rat colon, cannulated at both ends and infused with saline to increase intraluminal pressure. As pressure increases, the nerve signal rises, indicating its stimulation.
02:43
Fluorescence Imaging of Bacterial Colonization in Mouse Kidney Tissue
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2026
Source: Behera, R. K., et al. A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues. J. Vis. Exp. (2019)This video demonstrates a method for preparing infected mouse kidney tissue, staining DNA, and detecting bacterial gene expression using dual fluorescent reporters. Red marks all bacteria, and green highlights infection-responsive cells.
03:23
Assessment of Chemical-Induced Colitis via Immunohistochemical Analysis of Colon Tissue Sections
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2025
This video demonstrates the assessment of chemical-induced colitis via immunohistochemical staining of mouse colon tissue. In a stained tissue section, the presence of a large number of immune-responsive cells in the damaged tissue represents the infiltration of immune cells during colitis.
03:41
Harvesting Peritoneal Fat Depots: A Technique to Study Ovarian Cancer Colonization of Peritoneal Adipose Tissues in Mouse Models
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2023
In this video, we use a euthanized female mouse model bearing ovarian cancer. We then describe a protocol to identify and extract fat depots or adipose tissues associated with the five primary peritoneal organs to study metastatic cancer cell colonization.
FAQs
Persistence depends on a sequence of linked events rather than movement alone. Cells or microorganisms must reach a tissue, attach to local structures, adjust to available nutrients and environmental conditions, and interact with extracellular matrix components and immune defenses. Examining these stages helps researchers identify where spread is supported, restricted, or converted into longer-lasting residence.
Extracellular matrix components provide local structures with which invading or resident cells may interact, while immune defenses can influence whether those cells remain, are limited, or are removed. Studying both influences together connects physical tissue organization with host responses, helping explain differences in colonization patterns and tissue outcomes during infection, symbiosis, or disease.
Duration reflects how well cells or microorganisms adapt to local nutrient and environmental conditions while maintaining interactions with tissue structures and immune defenses. Colonization may remain temporary when these conditions do not support persistence, or continue when local conditions and host interactions favor residence. Comparing these situations clarifies why similar organisms can produce different tissue outcomes.
A practical investigation follows the sequence of events: examine movement into the tissue, attachment to local structures, adaptation to nutrients and environmental conditions, and interactions with extracellular matrix components and immune defenses. Researchers then relate these observations to tissue responses, spread, and persistence. This workflow organizes colonization as a process with distinguishable stages rather than a single endpoint.
Researchers examine it when they need to understand infection, symbiosis, development, or disease. The same framework can reveal how organisms spread through tissues, how host tissues respond, and why colonization becomes temporary or persistent. It therefore supports both studies of harmful interactions and investigations of relationships in which host and microorganism coexist.
Tracking colonization connects the presence and persistence of cells or microorganisms with changes in host tissues. Researchers can use this relationship to investigate how organisms spread, how tissue responses develop, and whether colonization remains localized or continues over time. These insights also inform strategies intended to limit harmful colonization in studies of tissue injury and disease progression.