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Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
DOI:

06:47 min

January 28, 2021

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Chapters

  • 00:05Introduction
  • 00:58Isolation of Plasma Lipoproteins
  • 03:00Results: Analysis of Lipoproteins and their Compositional Apolipoproteins Isolated from Rabbits on a Normal and High Cholesterol Diet
  • 05:32Conclusion

Summary

Automatic Translation

Several methods have been used for analyzing plasma lipoproteins; however, ultracentrifugation is still one of the most popular and reliable methods. Here, we describe a method regarding how to isolate lipoproteins from plasma using sequential density ultracentrifugation and how to analyze the apolipoproteins for both diagnostic and research purposes.

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