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JoVE Journal
Biology

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Assaying the Kinase Activity of LRRK2 in vitro
 

Assaying the Kinase Activity of LRRK2 in vitro

Article DOI: 10.3791/3495
January 18th, 2012

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Summary January 18th, 2012

Leucine Rich Repeat Kinase 2 is a large multidomain kinase, mutations in which are the most common genetic cause of Parkinson's disease. Analysis of the kinase activity of this protein has proven to be a crucial tool in understanding the biology and dysfunction of this protein. In this paper, in vitro assaying of the kinase activity of LRRK2 and a selection of its mutants is described, providing an experimental system to examine phosphorylation of putative substrates and potential dysfunction of LRRK2 in disease.

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