Jcat T Cells
Jcat T Cells - Related Videos
05:14
Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo
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2025
Given the limited xenograft models available to study interactions between human CART and myeloid cells, we established in vitro and in vivo models to understand the impacts of human macrophages on CART cells. Findings can potentially be generalized to evaluate macrophage roles in the tumor microenvironment and test macrophage-targeted immunotherapies.
08:04
In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function
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Cited by 23 •
2019
Here, we describe an in vitro co-culture method to recursively challenge tumor-targeted T cells, which allows for phenotypic and functional analysis of antitumor T cell activity.
10:04
Tractable In Vivo Reprogramming of Tumor Cells to Type 1 Conventional Dendritic Cell-like Cells
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2025
This protocol describes the in vivo reprogramming of mouse cancer cells into type 1 dendritic-like cells within the tumor microenvironment through enforced expression of the transcription factors PU.1, IRF8, and BATF3.
11:26
Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
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Cited by 8 •
2017
Here, we present a combinatorial approach for classifying neuronal cell types prior to isolation and for the subsequent characterization of single-cell transcriptomes. This protocol optimizes the preparation of samples for successful RNA Sequencing (RNA-Seq) and describes a methodology designed specifically for the enhanced understanding of cellular diversity.
![Dynamic Imaging of Chimeric Antigen Receptor T Cells with [<sup>18</sup>F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F62334.jpg%3Fv%3D2026-09-01%252021%253A51%253A00&w=750&q=75)
09:34
Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography
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Cited by 8 •
2022
This protocol describes the methodology for non-invasively tracking T cells genetically engineered to express chimeric antigen receptors in vivo with a clinically available platform.
FAQs
T-cell receptor stimulation starts linked intracellular events rather than a single endpoint. Protein phosphorylation can alter signaling proteins, calcium mobilization provides another intracellular signal, and transcription-factor activation connects these events to gene regulation. Measuring these stages separately helps investigators determine where an experimental compound or genetic modification changes the response, rather than observing only a final cellular effect.
These measurements represent different levels of the signaling response. Protein phosphorylation indicates changes within signaling pathways, calcium mobilization records an intracellular response to stimulation, and transcription-factor activation reflects control of gene regulation. Examining them together can help connect an early signaling event with later changes in gene expression or cellular behavior.
Researchers can compare stimulated cells with cells exposed to an experimental compound or genetic modification and then examine changes in signaling or gene expression. A difference in phosphorylation, calcium mobilization, transcription-factor activity, or cellular behavior can indicate that the tested condition influences a particular part of the immune-response pathway. This supports pathway-focused investigation under controlled conditions.
A study can begin by maintaining the cells under controlled laboratory conditions, applying a T-cell receptor stimulus, and measuring selected signaling or gene-regulation outcomes. Investigators may then introduce an experimental compound or genetic modification and compare the resulting response with an appropriate stimulated condition. The measured endpoints can include phosphorylation, calcium mobilization, transcription-factor activity, gene expression, or cellular behavior.
They may use this model when they want to examine whether a compound changes T-cell activation or the molecular pathways associated with it. After stimulation, researchers can assess effects on intracellular signaling, transcription-factor activation, gene expression, or cellular behavior. This controlled approach can support early evaluation of compounds relevant to immune regulation, inflammation, infection, or therapeutic development.
Their accessibility and reproducibility allow investigators to study immune-cell responses under controlled conditions before examining broader biological questions. Findings from signaling and gene-regulation experiments can inform research on inflammation, infection, and therapeutic development. The model therefore provides a practical experimental link between fundamental questions about T-cell recognition and applied studies of immune regulation.