Laser Power Control

Laser power control is the regulation of a laser’s optical output to deliver a precise amount of light for a defined experiment or biological application. It works by adjusting the beam through methods such as variable attenuation, electronic modulation, or feedback stabilization, while accounting for exposure time, wavelength, and sample sensitivity. In biology, controlled laser power supports fluorescence imaging, confocal microscopy, optical trapping, and laser-based manipulation by improving signal quality and reducing photobleaching, phototoxicity, and tissue damage. Reliable power control also strengthens quantitative measurements, enables reproducible comparisons across samples, and helps researchers balance imaging detail with cellular viability.

Laser Power Control - Related Videos

Education

JoVE Core - Electrical Engineering

Control of Power Flow

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2024

There are several methods to control power flow in power systems: Prime mover and excitation control of generators Switching of shunt capacitor banks, shunt reactors, and static var systems Control of tap-changing and regulating transformers A simple generator in the system is represented by its Thevenin equivalent circuit, which represents its model operating under balanced steady-state conditions. The key parameters include the generator terminal voltage Vt, the excitation voltage Eg,...

Research

JoVE EoE - Neuropathology

Inducing Targeted Neuronal Injury Using a High-Power Laser in a Drosophila Larva

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2025

This video demonstrates a method for inducing targeted neuronal injury in Drosophila larvae using a high-power laser. After positioning the larva under a microscope, a low-power laser scans for fluorescent neurons, and then the laser power is increased to induce localized damage. The injury is confirmed by increased fluorescence and a crater at the injury site.

Engineering Platform and Experimental Protocol for Design and Evaluation of a Neurally-controlled Powered Transfemoral Prosthesis

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Cited by 15 •

2014

Neural-machine interfaces (NMI) have been developed to identify the user's locomotion mode. These NMIs are potentially useful for neural control of powered artificial legs, but have not been fully demonstrated. This paper presented (1) our designed engineering platform for easy implementation and development of neural control for powered lower limb prostheses and (2) an experimental setup and protocol in a laboratory environment to evaluate neurally-controlled artificial legs on patients with...

Automation of Mode Locking in a Nonlinear Polarization Rotation Fiber Laser through Output Polarization Measurements

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Cited by 2 •

2016

A protocol to detect and automate mode locking in a pre-adjusted nonlinear polarization rotation fiber laser is presented. The detection of a sudden change in the output polarization state when mode locking occurs is used to command the alignment of an intra-cavity polarization controller in order to find mode-locking conditions.

Control of Cell Geometry through Infrared Laser Assisted Micropatterning

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2021

The protocol presented here enables automated fabrication of micropatterns that standardizes cell shape to study cytoskeletal structures within mammalian cells. This user-friendly technique can be set up with commercially available imaging systems and does not require specialized equipment inaccessible to standard cell biology laboratories.

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