Legume Symbiosis

Legume symbiosis is a mutualistic association between leguminous plants and nitrogen-fixing soil bacteria, typically rhizobia, that improves plant nutrition and soil fertility. Chemical signals from legume roots, including flavonoids, activate bacterial Nod factors, initiating root-hair infection and the formation of specialized nodules where bacterial cells differentiate into bacteroids; nitrogenase then converts atmospheric nitrogen into ammonia using energy supplied by plant-derived carbon. This process reduces the plant’s dependence on soil nitrogen and supports growth in nitrogen-poor environments, while benefiting agriculture through natural fertilization, crop rotation, reduced synthetic fertilizer use, and improved ecosystem nutrient cycling.

Legume Symbiosis - Related Videos

Education

JoVE Core - Biology

Symbiosis

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2026

Symbiotic relationships are long-term, close interactions between individuals of different species that affect the distribution and abundance of those species. When a relationship is beneficial to both species, this is called mutualism. When the relationship is beneficial to one species but neither beneficial nor harmful to the other species, this is called commensalism. When one organism is harmed to benefit another, the relationship is known as parasitism. These types of relationships often...

Research

JoVE Journal - Biology
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Layers of Symbiosis - Visualizing the Termite Hindgut Microbial Community

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Cited by 1 •

2007

Jared Leadbetter takes us for a nature walk through the diversity of life resident in the termite hindgut - a microenvironment containing 250 different species found nowhere else on Earth. Jared reveals that the symbiosis exhibited by this system is multi-layered and involves not only a relationship between the termite and its gut inhabitants, but also involves a complex web of symbiosis among the gut microbes themselves.

Single-plant, Sterile Microcosms for Nodulation and Growth of the Legume Plant Medicago truncatula with the Rhizobial Symbiont Sinorhizobium meliloti

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Cited by 9 •

2013

Growth of Medicago truncatula plants in symbiosis with the nitrogen-fixing bacteria Sinorhizobium meliloti in individual, sterile microcosms made from standard laboratory plates permits frequent examination of root systems and nodules without compromising sterility. Plants can be maintained in these growth chambers for up to 9 weeks.

Protocols for Obtaining Zygotic and Somatic Embryos for Studying the Regulation of Early Embryo Development in the Model Legume Medicago truncatula

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Cited by 2 •

2015

The goal is to illustrate that the model legume Medicago truncatula can be readily utilized to investigate the regulation of early plant embryogenesis to complement the non-legume Arabidopsis model. Pod morphology is linked to zygotic embryogenesis stages and a protocol to collect embryos using tissue culture is also provided.

Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions

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2025

This article describes a protocol for generating arbuscular mycorrhizal (AM) fungi inoculum to investigate AM-enhanced salt stress tolerance in rice.

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