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Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake
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1Molecular Neuropharmacology and Genetics Laboratory, Lundbeck Foundation Center for Biomembranes in Nanomedicine, Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences,University of Copenhagen, 2Laboratory of Neuropsychiatry, Psychiatric Center Copenhagen and Department of Neuroscience and Pharmacology,University of Copenhagen
Chapters
- 00:05Title
- 01:00Synaptosomal Preparation
- 03:22Uptake Experiment
- 07:00High-performance Liquid Chromatography Analysis
- 09:19Results: Representative Results from a Synaptosomal DA Uptake Experiment of Four C57BI/6 Wild Type Mice from Drd1a-cre Mouse Strain
- 10:40Conclusion
Synaptosomal dopamine uptake and high-performance liquid chromatography analysis represent experimental tools to investigate dopamine homeostasis in mice by assessing the function of the dopamine transporter and levels of dopamine in striatal tissue, respectively. Here we present protocols to measure dopamine tissue content and assess the functionality of the dopamine transporter.
