Lis-1 Insertion
Lis-1 Insertion - Related Videos
11:39
Peripheral Intravenous Catheter Insertion
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2023
Source: Madeline Lassche, MSNEd, RN and Katie Baraki, MSN, RN, College of Nursing, University of Utah, UT The purpose of peripheral intravenous catheter (PIV) insertion is to infuse medications, perform intravenous (IV) fluid therapy, or inject radioactive tracers for special examination procedures. Placing a PIV is an invasive procedure and requires the use of an aseptic, no-touch technique. Common IV venipuncture sites are the arms and hands in adults and the feet in children. According to...
10:32
Transvaginal Mesh Insertion in the Ovine Model
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Cited by 11 •
2017
This protocol describes mesh implantation in the ovine rectovaginal septum using a single vaginal incision technique, with and without the trocar-guided insertion of anchoring arms.
20:59
Large Insert Environmental Genomic Library Production
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Cited by 18 •
2009
Construction of a fosmid library with environmental genomic DNA isolated from the vertical depth continuum of a seasonally hypoxic fjord is described. The resulting clone library is picked into 384-well plates and archived for downstream sequencing and functional screening by the application of an automated colony picking system.
08:14
Central Venous Catheter Insertion: Subclavian Vein
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2023
Source: James W Bonz, MD, Emergency Medicine, Yale School of Medicine, New Haven, Connecticut, USA Central venous access is necessary in a multitude of clinical situations for hemodynamic monitoring, medication delivery, and blood sampling. There are three veins in the body that are accessed for central venous cannulation: the internal jugular, the subclavian, and the femoral vein. Central venous access via the subclavian vein has several advantages over other possible locations. The subclavian...
10:45
Central Venous Catheter Insertion: Femoral Vein
0 Views •
2023
Source: James W Bonz, MD, Emergency Medicine, Yale School of Medicine, New Haven, Connecticut, USA Central venous access is necessary in a multitude of clinical situations, including vascular access, vasopressor and caustic medication delivery, central venous pressure monitoring, volume resuscitation, total parental nutrition, hemodialysis, and frequent phlebotomy. There are three veins in the body that are accessed for central venous cannulation: the internal jugular, the subclavian, and the...
FAQs
The carrier construct does more than hold the LIS1 sequence: it places the gene beside regulatory elements that permit its expression. This arrangement supports transcription into RNA and subsequent translation into protein. Researchers can also incorporate a tag or engineered mutation, making it possible to examine LIS1 expression or evaluate the effects of altered protein forms in cells.
LIS1 acts as a conserved regulator of cytoplasmic dynein, the motor protein responsible for moving cargo along microtubules. Consequently, changing LIS1 expression can provide insight into processes that depend on dynein, including intracellular transport and spindle organization. These connections make LIS1 insertion useful for linking a molecular change to broader cellular functions.
Engineered LIS1 variants allow researchers to examine how particular regions of the protein contribute to dynein regulation. Comparing an altered sequence with the corresponding unmodified form can associate a domain with changes in LIS1-related activity. This approach is especially useful when the goal is to distinguish the effects of a specific structural alteration from the general presence of LIS1.
A basic strategy places the LIS1 gene sequence into either a plasmid or a cell genome. The inserted DNA is arranged with regulatory elements in a carrier construct so that cells can transcribe and translate the sequence. Depending on the experimental design, the construct may also contain a tag or mutation to produce a modified LIS1 protein.
Researchers use this strategy when they need to examine how LIS1 expression affects cellular function. Relevant applications include studying microtubule-dependent transport, cell division, and the organization of cellular structures supported by dynein. The approach can also help investigate how altered LIS1 forms influence these processes, extending the analysis beyond the unmodified gene sequence.
LIS1 insertion provides a way to investigate cellular mechanisms associated with developmental disorders by examining LIS1 expression and engineered variants. Because LIS1 regulates cytoplasmic dynein, experiments can connect altered protein behavior with microtubule-dependent transport, spindle organization, and neuronal migration. This places gene-level manipulation within a broader biological context involving development and cellular organization.