Liver Cells
Liver Cells - Related Videos
12:35
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
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Cited by 14 •
2011
The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.
12:06
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
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Cited by 22 •
2011
Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.
03:06
Developing a Humanized Mouse Model with Human Liver Cells and Immune Cells
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2025
This video demonstrates a method for generating a dual humanized mouse model with a human immune system and liver cells. It involves transplanting human liver cells and hematopoietic stem and progenitor cells into a transgenic immunodeficient mouse via intrasplenic injection, leading to engraftment of liver cells and development of human immune cells.
11:44
Generation of a Humanized Mouse Liver Using Human Hepatic Stem Cells
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Cited by 3 •
2016
Here, we present a novel humanized mouse liver model generated in Alb-toxin receptor mediated cell knockout (TRECK)/SCID mice following the transplantation of immature and expandable human hepatic stem cells.
09:24
Decellularization and Recellularization of Whole Livers
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Cited by 39 •
2011
Perfusion decellularization is a novel technique to produce whole liver scaffolds that retains the organ's extracellular matrix composition and microarchitecture. Herein, the method of preparing whole organ scaffolds using perfusion decellularization and subsequent repopulation with hepatocytes is described. Functional and transplantable liver grafts can be generated using this technique.
FAQs
Hepatocytes coordinate several linked tasks by processing nutrients, storing energy, modifying drugs and toxins, producing bile, and synthesizing plasma proteins. These activities affect both metabolism and the composition of blood reaching other tissues. Studying this coordination helps biologists connect cellular activity with whole-organ functions such as digestion, chemical clearance, and maintenance of internal conditions.
Kupffer cells and stellate cells contribute different forms of support around hepatocytes. Kupffer cells are associated with immune defense, whereas stellate cells help maintain tissue structure. Considering these populations together is important because liver performance depends not only on hepatocyte chemistry but also on defensive and structural support. This cellular organization provides context for investigating fibrosis and tissue repair.
Enzyme-driven reactions allow liver cells to modify drugs and toxins after those substances enter circulation. The key biological question is how these reactions alter the substances while the cells also regulate blood chemistry. Examining this activity is especially relevant to drug-toxicity research, where cellular processing can be evaluated alongside the liver’s broader metabolic and detoxification functions.
The regenerative capacity of liver cells makes them useful for studying how damaged tissue may be maintained or repaired. In biology, regeneration links cellular behavior with tissue-level recovery rather than representing a single metabolic task. Research on this property can therefore contribute to investigations of tissue repair and help explain why liver organization matters when assessing injury or disease.
Liver-cell research is applied to several biomedical questions, including drug toxicity, viral disease, fibrosis, and tissue repair. Each area emphasizes a different outcome: chemical effects, disease-related changes, structural scarring, or recovery of tissue. This range makes liver cells valuable experimental subjects for connecting normal physiology with pathological processes and repair mechanisms.
Studying liver cells provides a biological framework for understanding how specialized cell populations collectively regulate the internal environment. Hepatocyte activity influences nutrients, energy storage, bile production, and plasma proteins, while supporting cells contribute immune and structural functions. Researchers can use this combined perspective to relate cellular organization to blood chemistry, digestion, detoxification, and tissue maintenance.