Long-term Repopulating Cells
Long-term Repopulating Cells - Related Videos
09:43
Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development
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Cited by 17 •
2015
This protocol describes decellularization of Sprague Dawley rat kidneys by antegrade perfusion of detergents through the vasculature, producing acellular renal extracellular matrices that serve as templates for repopulation with human renal epithelial cells. Recellularization and use of the resazurin perfusion assay to monitor growth is performed within specially-designed perfusion bioreactors.
09:13
Study of Viral Vectors in a Three-dimensional Liver Model Repopulated with the Human Hepatocellular Carcinoma Cell Line HepG2
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Cited by 7 •
2016
The recellularized extracellular matrix of a decellularized rat liver can be used as a humanized, three-dimensional ex vivo model to study the distribution and transgene expression of a virus or viral vector.
06:41
Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells
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2023
We present a step-by-step protocol for the isolation of long-term hematopoietic stem cells (LT-HSCs) and short-term HSCs (ST-HSCs) using the Hoxb5 reporter system.
03:41
In Vitro Calcium Imaging in Short-Term and Long-Term Rat Hippocampal Cultures
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2025
Source: Calvo-Rodríguez, et al. Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons. J. Vis. Exp. (2015). This video demonstrates the use of fluorescence imaging to measure cytosolic calcium levels in short-term and long-term rat hippocampal cultures. By comparing young and aged neurons, it highlights how increased L-type voltage-gated calcium channel density and prolonged activation in aged neurons result in calcium overloading, a hallmark of neuronal aging.
FAQs
Their maintenance depends on balancing self-renewal with the generation of differentiated progeny. Asymmetric division allows one daughter cell to retain stem-cell properties while another enters differentiation. Symmetric division can instead expand the stem-cell pool or generate progeny, depending on the outcome. This balance supports continuing blood production without eliminating the repopulating-cell reserve.
Specialized bone marrow niches provide the anatomical setting in which hematopoietic repopulating cells reside. Their location is therefore an important consideration when researchers study tissue maintenance, stem-cell behavior, or changes associated with aging and disease. Examining these cells in relation to their niche helps connect cellular activity with the broader organization of blood production.
Durable multilineage reconstitution tests whether transplanted cells can generate several blood-cell lineages over an extended period. A result that persists across multiple lineages indicates more than temporary production of a limited cell type. For this reason, the transplantation outcome serves as a functional measure of stem-cell potency rather than relying only on cell identification.
Aging, genetic mutations, and disease can alter the regenerative behavior of long-term repopulating cells and the tissues they support. Researchers investigate these influences to determine how normal blood-cell production changes and how regeneration becomes impaired. Such studies also help establish experimental models for examining the biological basis of hematopoietic disorders.
In a transplantation-based evaluation, researchers determine whether transferred cells produce blood cells from multiple lineages and sustain that production over an extended period. Durable reconstitution provides the key functional readout. This approach allows investigators to compare stem-cell potency and to identify whether experimental conditions preserve the capacity for long-term hematopoietic regeneration.
They are useful when a study requires a functional assessment of stem-cell potency, an investigation of tissue maintenance, or an analysis of altered regeneration. Researchers also apply them to questions involving aging, genetic mutations, and disease. In hematopoietic biology, these cells connect basic studies of stem-cell behavior with transplantation strategies and disorder models.
Studies of long-term repopulating cells can reveal whether blood production remains sustained, whether multiple lineages are generated, and how regenerative capacity changes under disease-related conditions. Their use in experimental models gives researchers a way to examine hematopoietic disorders through a stem-cell function that can be tested by durable transplantation outcomes.