Lsm 510 Meta Nlo
Lsm 510 Meta Nlo - Related Videos
01:09
Directing Effect of Substituents: meta-Directing Groups
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2025
Substituents on the benzene ring that direct an incoming electrophile to undergo substitution at the meta position are called meta directors. All meta directors either have a positive charge on the atom directly bonded to the ring or a partial positive charge. These groups function by withdrawing electrons from the ring through inductive and resonance effects. Consider the carbocation intermediates formed upon the addition of an electrophile on nitrobenzene at the ortho, meta, and para...
04:34
Meta-Analysis of the Effectiveness and Safety of Shugan Jieyu Capsules for the Treatment of Insomnia
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Cited by 3 •
2023
The presented protocol describes an investigation strategy and systematic review to evaluate the clinical effectiveness and safety of Shugan Jieyu capsules for treating insomnia.
01:13
meta-Directing Deactivators: –NO2, –CN, –CHO, –CO2R, –COR, –CO2H
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2023
All meta-directing substituents are deactivating groups. These substituents withdraw electrons from the aromatic ring, making the ring less reactive toward electrophilic substitution. For example, the nitration of nitrobenzene is 100,000 times slower than that of benzene because of the deactivating effect of the nitro group. The first step in an electrophilic aromatic substitution is the addition of an electrophile to form a resonance-stabilized carbocation. The energy diagrams for the...
08:48
Quantitation of γH2AX Foci in Tissue Samples
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Cited by 7 •
2010
Quantitation of DNA double-strand breaks on the basis of γH2AX foci has become an invaluable tool, particularly in radiation biology, for the evaluation of tissue radiosensitivity and effects of radiation modifying compounds. Here we demonstrate the use of an immunofluorescence assay for quantitation of γH2AX foci in tissue samples.
06:26
Meta-analysis of Voxel-Based Neuroimaging Studies using Seed-based d Mapping with Permutation of Subject Images (SDM-PSI)
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Cited by 100 •
2019
We detail how to conduct a meta-analysis of voxel-based neuroimaging studies using Seed-based d Mapping with Permutation of Subject Images (SDM-PSI).
FAQs
Spectral separation allows the META detector to distinguish fluorescence signals by their emission wavelengths, even when multiple fluorophores contribute to the same image. This capability helps assign colors to different labeled structures rather than treating overlapping emissions as one signal. In biological samples, that improves interpretation of molecular localization and supports multicolor analysis within the same specimen.
The near-infrared pulsed light used for nonlinear excitation generates signals from focal regions within the specimen. Restricting signal generation to these regions supports optical sectioning and can help imaging at greater tissue depths. This is especially relevant when researchers need information from inside cells, tissues, or developing organisms rather than only from their surfaces.
Image formation depends on scanning focused laser light across the specimen and recording the resulting optical signals. Because the nonlinear signal originates from focal regions, the system can separate information from different positions within the sample. Repeated spatial measurements therefore support optical sectioning and three-dimensional analysis of biological structures and their organization.
Combining spectral fluorescence detection with nonlinear optical excitation addresses two different imaging challenges at once. Wavelength-based separation helps resolve overlapping fluorophore signals, while focal nonlinear excitation contributes depth-resolved information. Together, these capabilities make it possible to examine several labeled components and their spatial relationships in complex biological samples.
A basic workflow scans focused laser light across the specimen while collecting optical signals from the illuminated regions. The META detector separates fluorescence according to emission wavelength, and nonlinear excitation produces signals from focal regions when near-infrared pulsed light is used. The collected information can then support multicolor visualization, optical sectioning, and three-dimensional analysis.
Data from the LSM 510 META NLO can reveal cellular organization, molecular localization, and dynamic biological processes. Spectral information helps associate signals with different fluorophores, while optical sectioning and three-dimensional analysis place those signals within the specimen. The resulting observations support interpretation of how labeled components are arranged and change in biological systems.
The platform is suited to imaging cells, tissues, and developing organisms when researchers need detailed spatial information. Its multicolor capability can separate fluorescence contributions, and its nonlinear imaging capability can extend observations into greater tissue depths. These features support studies of structure, molecular localization, and biological change across different levels of organization.