Production and Detection of Reactive Oxygen Species (ROS) in Cancers

Danli Wu; Patricia Yotnda

doi:10.3791/3357

Production and Detection of Reactive Oxygen Species (ROS) in Cancers

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Medicine

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JoVE Journal Medicine

Production and Detection of Reactive Oxygen Species (ROS) in Cancers

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71,134 Views

07:17 min

November 21, 2011

DOI: 10.3791/3357-v

Danli Wu¹, Patricia Yotnda¹

¹Center for Cell and Gene Therapy,Baylor College of Medicine

Here we propose simple methods to test and evaluate the presence of reactive oxygen species in cells.

The aim of this experiment is to detect the presence of reactive oxygen species in cell cultures. This is accomplished by first detecting reactive oxygen species using a fluorescent dye. Next, the levels of nitric oxide in cells are measured using a colormetric assay.

Finally, the effect of reactive oxygen species is determined by detecting the reduction or oxidation of glutathione using a luminescent assay. My name is Danu. I'm a poster in the lab of Patrizia Yoda, and I will be demonstrating this procedure.

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