Host Organism
Host Organism - Related Videos
04:09
An Ex Vivo Gut Organ Culture System to Study Host-Microbiota Interactions
0 Views •
2025
In this video, we describe the methodology for setting up an ex vivo gut organ culture system to study the interactions between mouse intestinal tissue fragments and specific gut bacteria. The device consists of two pairs of input-output ports, the first connecting the intestinal tissue lumen and supplying the specific bacterial suspension into it, and the second continuously supplying media to the device's wells to maintain ex vivo tissue viability.
10:40
The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies
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Cited by 15 •
2017
The ex vivo organ culture allows investigation of biological processes in the context of the intact tissue architecture. Here, we introduce a method of ex vivo culture of the mouse colon, which can be used to study innate immunity and antimicrobial host defense in the intestine.
12:16
Porphyromonas gingivalis as a Model Organism for Assessing Interaction of Anaerobic Bacteria with Host Cells
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Cited by 17 •
2015
This article presents two protocols: one to measure anaerobic bacteria that can successfully invade and survive within the host, and the other to visualize anaerobic bacteria interacting with host cells. This study can be applied to any cultivable anaerobe and any eukaryotic cell type.
05:27
An Intestinal Gut Organ Culture System for Analyzing Host-Microbiota Interactions
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Cited by 5 •
2021
This article presents a unique method for analyzing host-microbiome interactions using a novel gut organ culture system for ex vivo experiments.
02:05
Establishing a Binary Culture of Saccharibacteria with Host Bacteria
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2025
Source: Collins, A. J., et al., Establishing Stable Binary Cultures of Symbiotic Saccharibacteria from the Oral Cavity. J. Vis. Exp. (2021)This video demonstrates the establishment and maintenance of a binary culture of Saccharibacteria and its host bacterium by sequentially co-incubating them in host-specific growth medium.
FAQs
Host physiology and immune defenses help determine whether an associated biological agent can persist and how the interaction develops. Physiological conditions can provide or restrict access to nutrients, shelter, and cellular compatibility, while immune responses may alter those conditions. Examining both sides helps explain differences in survival, growth, reproduction, infection, or coexistence.
Nutrients, shelter, and compatible cellular or environmental conditions determine what the associated organism or agent can obtain from the host. When these conditions support survival, growth, or reproduction, the association can persist; when host physiology changes them, the outcome may shift. This makes resource access and compatibility important variables in studying host-associated biology.
Host organisms can be examined in relation to parasites, pathogens, symbionts, or commensals, but the relevant biological question changes with the association. Researchers may focus on survival, growth, reproduction, infection, disease transmission, or coexistence, depending on the system. This framing prevents host effects from being treated as identical across all associated organisms or agents.
A change in one host organism can influence associated communities and may extend to broader ecosystem effects. Because hosts provide conditions that shape survival, growth, reproduction, and interaction outcomes, altered host physiology or defenses can change relationships within the system. Studying these connections helps link individual biological responses with community-level and ecological consequences.
A host organism model system allows researchers to examine biological mechanisms in a living context and test interventions against those mechanisms. It can also help assess how changes in one organism affect an associated organism or agent. This approach connects experimental manipulation with outcomes involving infection, disease transmission, symbiosis, or other host-associated relationships.
Within biotechnology and experimental biology, researchers use host-based systems to examine biological mechanisms and test interventions. The host provides a context in which an intervention can be evaluated alongside relevant physiology and immune defenses. These studies can reveal how changing the system affects an associated organism or agent, rather than examining that organism or agent in isolation.
Host organisms connect several areas of biology because they are central to studying infection, disease transmission, symbiosis, and ecological relationships. Research can examine effects within the host, changes in associated communities, and consequences for broader ecosystems. This range makes host-based investigation relevant to both biological mechanisms and the larger context in which those mechanisms operate.