Metabolic Enzymes
Metabolic Enzymes - Related Videos
05:41
Metabolic Profile Analysis of Zebrafish Embryos
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Cited by 42 •
2013
Zebrafish represent a powerful vertebrate model that has been under-utilised for metabolic studies. Here we describe a rapid way to measure the in vivo metabolic profile of developing zebrafish that allows the comparison of different mitochondrial function parameters between genetically or pharmacologically manipulated embryos, thereby increasing the applicability of this organism.
02:34
Enzymes
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2020
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions. Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
05:33
Using Modified Synthetic Oligonucleotides to Assay Nucleic Acid-Metabolizing Enzymes
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2024
Here, a protocol for assaying nucleic acid metabolizing enzymes is presented, using examples of ligase, nuclease, and polymerase enzymes. The assay utilizes fluorescently labeled and unlabeled oligonucleotides that can be combined to form duplexes mimicking RNA and/or DNA damages or pathway intermediates, allowing for the characterization of enzyme behavior.
10:19
An Introduction to Cell Metabolism
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2023
In cells, critical molecules are either built by joining together individual units like amino acids or nucleotides, or broken down into smaller components. Respectively, the reactions responsible for this are referred to as anabolic and catabolic. These reactions require or produce energy typically in the form of a “high-energy” molecule called ATP. Together, these processes make up “Cell Metabolism,” and are hallmarks of healthy, living cells.JoVE’s introduction to cell metabolism briefly...
00:52
What is Metabolism?
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2019
Overview Metabolism represents all of the chemical activity in a cell, including reactions that build molecules (anabolism) and those that break molecules down (catabolism). Anabolic reactions require energy, whereas catabolic reactions provide it. Thus, metabolism describes how cells transform energy through a variety of chemical reactions, which are often made more efficient with the help of enzymes. Metabolism Is the Sum of All of the Chemical Reactions That Take Place in an Organism
FAQs
Specificity comes from the match between a substrate and an enzyme’s active site. That interaction positions the substrate for a particular chemical transformation and lowers the activation energy, the energy barrier that otherwise slows the reaction. In metabolism, this selectivity helps direct compounds into defined steps of glycolysis, the citric acid cycle, or biosynthetic pathways.
Cofactor and substrate availability can determine how effectively a metabolic reaction proceeds. Cofactors support enzyme activity, while substrates provide the compounds on which reactions act. Because metabolic pathways are interconnected, changes in either factor can affect the movement of compounds through energy-producing or biosynthetic reactions and alter downstream cellular processes.
Temperature, pH, and regulatory signals can shift enzyme activity, allowing metabolism to respond to changing cellular conditions. These variables do not act in isolation: altered activity at one reaction can affect compound availability for subsequent pathway steps. Regulation therefore helps coordinate energy production, molecule building, and cellular maintenance rather than treating reactions as independent events.
Metabolic enzyme measurements can provide indicators of tissue health and disease by revealing changes in enzyme activity. Their interpretation becomes more informative when connected to the pathways under study, because altered activity may relate to energy use, biosynthesis, or broader cellular function. Such measurements help connect molecular reactions with the condition of tissues.
Studying metabolic enzymes helps explain inherited metabolic disorders, drug responses, and nutritional effects by relating these subjects to cellular reactions and pathway regulation. This perspective connects molecular activity with tissue health and disease while clarifying how cells obtain energy and build necessary molecules. It also helps organize observations across multiple steps of metabolism.
Metabolic enzymes support biotechnology research by providing insight into the chemical transformations that occur within biological systems. Examining their activity alongside pathways such as glycolysis, the citric acid cycle, and biosynthesis helps researchers understand how compounds are processed. This pathway-level knowledge supplies scientific context for investigating biological systems and their potential biotechnological uses.