Mouse Skin Imaging
Mouse Skin Imaging - Related Videos
09:08
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
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Cited by 59 •
2011
Magnetic resonance imaging (MRI) has become an increasingly popular tool for examining the phenotype of genetically altered mice. This article illustrates the methods necessary to achieve high-throughput phenotyping of genetically altered mice using multiple-mouse MRI.
07:51
Intravital Two-Photon Imaging of Touch Sensory Axon Morphology in Mouse Skin
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2025
This study presents an in vivo two-photon imaging approach for visualizing the structures of low-threshold mechanoreceptor (LTMR) axon terminals in the forepaw skin of mice. Using repeated, high-resolution imaging of individual axons, this method provides a new platform for studying the structure and function of the cutaneous sensory circuit during development and in adults.
08:29
Ex vivo Culture of Mouse Embryonic Skin and Live-imaging of Melanoblast Migration
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Cited by 11 •
2014
We describe the dissection and ex vivo culture of mouse embryonic skin. The culture system maintains an air-liquid interface across the tissue surface and allows imaging on an inverted microscope. Melanoblasts, a component of the developing skin, are fluorescently labeled allowing their behavior to be observed using confocal microscopy.
06:29
Inducing Ischemia-reperfusion Injury in the Mouse Ear Skin for Intravital Multiphoton Imaging of Immune Responses
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Cited by 15 •
2016
This protocol describes the induction of an ischemia-reperfusion (IR) model on mouse ear skin using magnet clamping. Using a custom-built intravital imaging model, we study in vivo inflammatory responses post-reperfusion. The rationale behind the development of this technique is to extend the understanding of how leukocytes respond to skin IR injury.
03:35
A Technique for Human Skin Grafting in a Mouse Model
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2025
This video demonstrates a method for grafting human skin onto an immunodeficient mouse with a suppressed neutrophil response. The method involves applying human split-thickness skin to the mouse's graft bed, sealing it, and covering it for recovery. Eventually, the human skin adheres to the mouse's skin, establishing vascularization and circulation, resulting in a successful skin xenograft.
FAQs
Reflected light reveals tissue features through light returned from the sample, whereas emitted light can arise from fluorescent labels or genetically encoded reporters. This distinction lets investigators visualize either structural organization or selected biological signals. Choosing between these signal sources helps align the imaging approach with the feature or activity being studied.
Fluorescent labels and genetically encoded reporters provide optical signals that identify particular cells, structures, or biological activities within mouse skin. Because the collected signal can be associated with a defined target or process, these tools help connect visible tissue changes with cellular behavior, including immune-cell movement or activity during disease-related studies.
Architecture and behavior provide complementary biological information. Imaging the epidermis, dermis, hair follicles, or blood vessels shows how tissue organization changes, while following cells reveals dynamic responses within that structure. Combining both views can link tissue-level alterations to cellular events, strengthening interpretation of processes such as inflammation, wound healing, and tumor development.
Living tissue supports observation of biological activity as it occurs in the mouse, including changes in cells or tissue over time. Preserved tissue instead provides a fixed record of skin structure and signals at the examined state. Using either condition depends on whether the study emphasizes dynamic behavior, tissue organization, or both.
A study generally begins by selecting the skin feature or biological activity of interest, such as follicles, vessels, immune cells, or a disease response. Investigators then use an appropriate optical or microscopic approach and collect reflected or emitted light from living or preserved tissue. The resulting images connect visible patterns with the biological question.
Mouse skin imaging supports questions about wound healing, inflammation, infection, tumor development, and hair-follicle cycling. It can also be used to examine drug delivery in skin. These applications allow researchers to relate disease or treatment-associated changes to tissue structure and cellular activity rather than evaluating either feature in isolation.
In biology, the approach helps researchers examine how skin cells, structures, and activities change during normal or disease-related processes. In therapy-focused work, it can show how a potential treatment relates to tissue changes or delivery within skin. This combination of structural and dynamic information supports investigation of disease mechanisms and evaluation of potential therapies in vivo.