A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Singapore Immunology Network (SIgN),Agency for Science, Technology and Research (A*STAR), Biopolis, 2Department of Microbiology and Immunology, Yong Loo Lin School of Medicine,National University of Singapore, 3Lee Kong Chian School of Medicine,Nanyang Technological University, 4Centenary Institute for Cancer Medicine and Cell Biology, 5Discipline of Dermatology,University of Sydney, 6Department of Dermatology,Royal Prince Alfred Hospital, 7LSI Immunology Programme,National University of Singapore, 8School of Biological Sciences,Nanyang Technological University
Chapters
- 00:05Title
- 00:53Mouse Anesthesia and Depilation
- 01:35Induction of Ischemia and Reperfusion Injury
- 02:31Placement of the Ear on the Imaging Platform
- 04:33Results: Neutrophil Recruitment in Ischemia-reperfusion Injury
- 05:20Conclusion
This protocol describes the induction of an ischemia-reperfusion (IR) model on mouse ear skin using magnet clamping. Using a custom-built intravital imaging model, we study in vivo inflammatory responses post-reperfusion. The rationale behind the development of this technique is to extend the understanding of how leukocytes respond to skin IR injury.
