Growth Assessment

Growth assessment in biology is the quantitative evaluation of how an organism, tissue, cell population, or culture changes in size, mass, cell number, or biomass over time. Researchers measure these changes under defined conditions using methods such as cell counting, weighing, imaging, or optical-density readings, then interpret the results through growth curves and calculated growth rates. In microbial and cell culture studies, measurements can distinguish lag, exponential, stationary, and decline phases. Growth assessment supports investigations of development, physiology, environmental responses, disease progression, and the effects of nutrients, drugs, or other experimental conditions.

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JoVE Journal - Biology

Growth Assays to Assess Polyglutamine Toxicity in Yeast

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Cited by 3 •

2012

This manuscript describes three complementary protocols for assessing the toxicity of polyglutamine (polyQ)-expansion proteins in the yeast Saccharomyces cerevisiae. These protocols can easily be modified to monitor the toxicity of other misfolded proteins in yeast.

Assessing Rotavirus Growth Curve by Plaque Assay

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2026

Source: Kadoya, S., Sano, D. Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus. J. Vis. Exp. (2019).This video demonstrates the use of a plaque assay to assess the rotavirus growth curve. The method involves infecting host cells with diluted virus, overlaying with agarose to restrict spread, and staining to visualize plaques formed by viral replication. By counting plaque-forming units at defined time points, the resulting growth curve reveals the lag, exponential, and...

Bioluminescence Imaging to Assess Tumor Growth in an Organotypic Mouse Brain Slice

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2025

Source: Ciraku, L., et. al. An Ex Vivo Brain Slice Model to Study and Target Breast Cancer Brain Metastatic Tumor Growth. J. Vis. Exp. (2021).This video demonstrates the imaging of luciferase-expressing tumor cells in an organotypic mouse brain slice. Luciferin is added to the medium, and the plate is placed inside the chamber of the in vivo imaging system. Parameters for image capture are adjusted, and the tumor's shape and size are defined. The bioluminescent signals emitted by the tumor...

Assessment of Intracellular Growth of Coxiella Mutants within Eukaryotic Cells

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2025

This video demonstrates a method for assessing the intracellular growth of GFP-tagged Coxiella mutants within cultured epithelial cells. The mutant phenotype is visualized using immunostaining and epifluorescence microscopy, allowing the study of the effect of the mutation on intracellular growth.

In Vitro Assessment of Aggregated Amyloid-β on Neuronal Growth Cone Collapse

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2025

This video demonstrates an in vitro assessment of neuronal growth cone collapse by amyloid-β (Aβ) aggregates. Treating the neurons with Aβ aggregates causes cytoskeletal destabilization, leading to the collapse of growth cones at the tip of the axons. The treated cells are compared with control cells to assess the extent of growth cone collapse.

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