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Engineering Cell-permeable Protein
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal Biology
Engineering Cell-permeable Protein
DOI:

21:08 min

December 28, 2009

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Chapters

  • 00:00Title
  • 01:38Introduction
  • 02:34Expression
  • 06:54Purification
  • 13:46Protein Transduction
  • 20:02Representative Outcomes/Conclusion

Summary

Automatic Translation

Protein transduction enables the direct delivery of biologically active proteins into cells. In contrast to conventional methods such as DNA transfection or viral transduction this non-invasive paradigm allows highly efficient cellular manipulation in a titratable manner circumventing cellular toxicity and the risk of oncogenic transformation by permanent genetic modification.

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