Organism Viability Testing
Organism Viability Testing - Related Videos
12:03
Viability Assays for Cells in Culture
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Cited by 39 •
2014
Therapeutic compounds are often first examined in vitro with viability assays. Blind cell counts by a human observer can be highly sensitive to small changes in cell number but do not assess function. Computerized viability assays, as described here, can assess both structure and function in an objective manner.
05:47
Counting and Determining the Viability of Cultured Cells
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Cited by 19 •
2008
Determining the number of cells in culture is important in standardization of culture conditions and in performing accurate quantitation experiments. In this video, we demonstrate how cells are counted using a hemacytometer.
02:38
Fluorescence-Based Viability Assessment of Oral Anaerobic Bacteria
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2026
Source: Calatayud Arroyo, M., et al. Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface. J. Vis. Exp. (2018)The video demonstrates a method to evaluate the viability of anaerobic oral bacteria using fluorescent dyes and flow cytometry. It begins with culturing bacteria in oxygen-free broth, followed by resuspending a single colony from a blood agar plate. After incubation and dilution, the culture is stained with membrane-permeable and...
08:29
A 3D-printed Chamber for Organic Optoelectronic Device Degradation Testing
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Cited by 1 •
2018
Here, we present a protocol for the design, manufacture, and use of a simple, versatile 3D-printed and controlled atmospheric chamber for the optical and electrical characterization of air-sensitive organic optoelectronic devices.
07:05
Viability of Bioprinted Cellular Constructs Using a Three Dispenser Cartesian Printer
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Cited by 6 •
2015
A Cartesian bioprinter was designed and fabricated to allow multi-material deposition in precise, reproducible geometries, while also allowing control of environmental factors. Utilizing the three-dimensional bioprinter, complex and viable constructs may be printed and easily reproduced.
FAQs
A membrane-integrity readout indicates whether cell boundaries remain intact, whereas metabolic activity indicates whether biochemical processes continue. These signals can therefore describe different aspects of specimen status rather than interchangeable measurements. Comparing them helps researchers interpret biological responses to drugs, toxins, environmental stress, or preservation conditions with greater specificity in cell culture and microbiology studies.
Growth and reproduction provide evidence that a specimen can continue functioning over time, not merely retain an intact structure or produce a short-term biochemical signal. Culture-based observations can therefore reveal whether microorganisms or other biological specimens recover and expand under suitable conditions. This information is especially useful when evaluating survival after stress, treatment, or preservation.
Viability stains assess a visible indicator associated with specimen status, while biochemical readouts measure chemical activity and optical methods detect measurable signals from the sample. Culture-based assays instead emphasize later growth or reproduction. Selecting among these approaches depends on whether the experiment needs a structural, biochemical, optical, or longer-term functional indication of survival.
A basic workflow identifies the biological specimen and the condition being evaluated, such as drug exposure, toxin exposure, environmental stress, or preservation. The researcher then selects an indicator, applies a viability stain, culture-based assay, or biochemical or optical method, and records the resulting readout. Comparing measurements across conditions allows survival to be quantified.
Researchers use these assessments when they need to determine how biological specimens respond to drugs, toxins, environmental stress, or preservation conditions. The results support work in cell culture, microbiology, toxicology, and biotechnology by providing evidence of survival or loss of functional capacity. They can also help evaluate whether a biological preparation remains suitable for continued research or development.
Viability measurements distinguish a change in biological response from a loss of living material that could otherwise confound interpretation. For example, a treatment-related result can be considered alongside membrane status, metabolic activity, growth, or reproduction. In biology experiments, this connection helps relate observed effects to specimen survival and supports more meaningful comparisons among exposure or preservation conditions.