Osmotic Pump Assembly

Osmotic pump assembly is the construction of a controlled-release device that delivers a solution or compound at a predictable rate, making it useful for sustained biological experiments and therapies. The device typically contains an osmotic agent and test solution separated from surrounding fluid by a semipermeable membrane; water enters by osmosis, generating pressure that steadily drives the contents through a small outlet. In biology, assembled osmotic pumps can administer drugs, hormones, or other research compounds over extended periods in cell, tissue, or animal studies. This approach reduces repeated dosing, supports consistent exposure, and improves the study of long-term physiological and pharmacological effects.

Osmotic Pump Assembly - Related Videos

Research

JoVE Journal - Medicine
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Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice

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Cited by 84 •

2015

Subcutaneous implantation of osmotic pumps provides a convenient approach for prolonged and consistent delivery of compounds. This approach has been used extensively to study both abdominal and thoracic aortic aneurysms in mice.

Research

JoVE EoE - Neurotherapeutics

Osmotic Pump-Based Neurotherapeutic Drug Delivery in a Demyelinated Mouse Model

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2025

Source: Wang, X. et. al., Osmotic Pump-based Drug-delivery for In Vivo Remyelination Research on the Central Nervous System. J. Vis. Exp. (2021)This video demonstrates the method for delivering neurotherapeutic drugs using an osmotic pump in a demyelinated mouse model. The controlled drug release promotes remyelination by activating myelin-producing cells in the corpus callosum.

Subcutaneous Neurotrophin 4 Infusion Using Osmotic Pumps or Direct Muscular Injection Enhances Aging Rat Laryngeal Muscles

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Cited by 7 •

2017

Here, we present a protocol to describe the use of neurotrophin 4 (NTF4) systemically and directly to remodel rat aging laryngeal muscles.

Continuous Drug Infusion System in Mouse Model: A Surgical Procedure to Implant Micro-osmotic Pump Infusion System in the Mouse Brain for Continuous Drug Delivery

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2025

In this video, we demonstrate the assembly of a micro-osmotic pump brain infusion system followed by its surgical implantation in the mouse skull. This system enables continuous delivery of potential drugs and facilitates the study of their effects on the brain and brain tissue.

Research

JoVE Journal - Medicine
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Neurodegeneration in an Animal Model of Chronic Amyloid-beta Oligomer Infusion Is Counteracted by Antibody Treatment Infused with Osmotic Pumps

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Cited by 11 •

2016

To study the effects of Aβo in vivo, we developed a model based on repeated hippocampal infusions of soluble Aβo coupled with continuous infusion of Aβo antibody (6E10) in the hippocampus using osmotic pumps to counteract the neurotoxic effect of Aβo.

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