Pet Membrane Slides
Pet Membrane Slides - Related Videos
22:00
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
0 Views •
Cited by 48 •
2010
Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic mesophases.
10:35
Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia
0 Views •
Cited by 4 •
2015
The method presented here uses simultaneous positron emission tomography and magnetic resonance imaging. In the cerebral hypoxia-ischemia model, dynamic changes in diffusion and glucose metabolism occur during and after injury. The evolving and irreproducible damage in this model necessitates simultaneous acquisition if meaningful multi-modal imaging data are to be acquired.
01:18
Design Example: Designing Water Slide
0 Views •
2025
When designing a water slide, controlling the speed of water flow is crucial for rider safety while maintaining an exciting experience. As water flows down the slide, gravity causes it to accelerate, with its speed at the bottom depending on the height from which it starts. The higher the slide, the more potential energy the water has at the top, which is converted into kinetic energy as it descends, increasing its speed. Bernoulli's principle determines the water's velocity along the slide.
06:14
In vitro Biofilm Formation in an 8-well Chamber Slide
0 Views •
Cited by 62 •
2011
This article describes the procedure for the formation and visualization of a bacterial biofilm grown within an 8-well chamber...
02:39
An In Vivo PET Imaging Technique to Detect Tumors in a Murine Model Using Radiolabeled Antibodies
0 Views •
2025
This video demonstrates a technique involving a radioimmunoconjugate for the in vivo imaging of a tumor in a mouse model. Upon injection of the radioimmunoconjugate, the antibodies of the conjugate bind to a specific membrane antigen on the cancer cells. The radiotracer of the conjugate emits positrons that interact with neighboring electrons to produce energy, which is detected by a positron emission tomography (PET) scanner to create an image of the tumor.
FAQs
The porous membrane creates two adjacent environments while preserving a shared soluble-factor pathway. Nutrients, signaling molecules, and other dissolved factors can diffuse through the pores, allowing cells on or near the membrane to respond to influences from the neighboring side. This arrangement helps investigators examine how spatial separation and molecular exchange affect cell behavior under controlled laboratory conditions.
Cells may migrate through the membrane in assays designed to examine movement or invasion. The pores provide a defined route between adjacent environments, allowing researchers to observe whether cells remain attached to the surface or pass through the porous region. This distinction makes the slide useful for comparing stationary cellular behavior with movement-related responses.
Because the membrane separates neighboring environments while allowing soluble factors to pass, it can help researchers examine barrier behavior in a cell-based setting. Cells attached to the surface provide the biological layer being observed, and microscopy can reveal how organization and transport-related interactions appear under assay conditions. This supports studies linking cellular barriers with tissue-level processes.
An experiment can focus on either cells remaining attached to the membrane or cells that migrate through its pores. That distinction lets the same support accommodate questions about cell growth and morphology as well as movement-related behaviors such as migration and invasion. The selected assay determines which location and cellular response becomes the main observation.
Cells are cultured on the membrane under the chosen laboratory conditions, then the slide can be prepared for staining and microscopy. Investigators examine the resulting preparation for features such as cell attachment, growth, morphology, or movement through the pores. This workflow links a controlled culture setup with direct visual assessment of cell behavior.
Staining and imaging can provide evidence about cell morphology, growth, and attachment to the membrane. In assays designed for movement, images can also show cells associated with migration or invasion through the porous region. These observations help connect visible cellular changes with the behavior being tested, rather than relying on the support alone.
In biology, these slides provide a model for examining how cells behave within an organized, partially separated environment. Their use connects cell-based observations with tissue organization and transport processes, making them relevant to cell biology, developmental studies, and disease modeling. The platform can therefore relate cellular behavior to broader biological structure and function.