Phosphorus-31 Mrs
Phosphorus-31 Mrs - Related Videos
10:49
Laboratory-determined Phosphorus Flux from Lake Sediments as a Measure of Internal Phosphorus Loading
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Cited by 18 •
2014
Lake eutrophication is a water quality issue worldwide, making the need to identify and control nutrient sources critical. Laboratory determination of phosphorus release rates from sediment cores is a valuable approach for determining the role of internal phosphorus loading and guiding management decisions.
08:46
Preparation and Reactivity of a Triphosphenium Bromide Salt: A Convenient and Stable Source of Phosphorus(I)
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Cited by 4 •
2016
The synthesis of a triphosphenium bromide salt is described and its use as a P+ transfer agent is outlined by reactions with an N-heterocyclic carbene and an anionic bisphosphine, yielding an NHC-stabilized P(I) cation and a P(I) containing zwitterion, respectively.
11:38
Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy
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Cited by 36 •
2010
Langendorff-mode isolated heart perfusion, in conjunction with 31P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.
13:31
Soil Nutrient Analysis: Nitrogen, Phosphorus, and Potassium
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2023
Source: Laboratories of Margaret Workman and Kimberly Frye - Depaul University In this experiment, three soil macronutrients are chemically extracted, combined with color-based reagents, then analyzed using color to determine the nutrient concentration present in the soil sample. Nitrogen, phosphorus, and potassium are the main components of soil fertilizer. These methods isolate each nutrient from the soil into a solution that can be analyzed using turbidity and color to determine the...
09:40
Phosphorus-31 Magnetic Resonance Spectroscopy: A Tool for Measuring In Vivo Mitochondrial Oxidative Phosphorylation Capacity in Human Skeletal Muscle
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Cited by 9 •
2017
This work demonstrates the feasibility of an in vivo phosphorus-31 magnetic resonance spectroscopy (31PMRS) technique to quantify mitochondrial oxidative phosphorylation (OXPHOS) capacity in human skeletal muscle.
FAQs
Chemical shift is the frequency difference that separates phosphorus-containing signals in a spectrum. In Phosphorus-31 MRS, those shifted signals allow ATP, phosphocreatine, inorganic phosphate, phosphomonoesters, and phosphodiesters to be distinguished within living systems. This separation turns one measurement into a biochemical profile, supporting interpretation of energy metabolism and membrane-related processes without disrupting the sample.
A strong magnetic field organizes the magnetic properties of phosphorus-31 nuclei, while radiofrequency excitation perturbs them. As the nuclei respond, they generate signals whose chemical shifts reflect the phosphorus-containing compounds present. Measuring these differences enables researchers to separate several metabolites in the same living system and examine biochemical states without removing or destroying tissue.
The measured spectrum can distinguish ATP, phosphocreatine, inorganic phosphate, phosphomonoesters, and phosphodiesters. ATP and phosphocreatine provide information relevant to cellular energy metabolism, whereas phosphomonoesters and phosphodiesters support assessment of membrane biology and turnover. Inorganic phosphate contributes to the metabolic profile, allowing several aspects of cellular function to be examined together.
By measuring phosphorus-containing metabolites in living tissue, 31P MRS can assess tissue bioenergetics and mitochondrial function alongside intracellular pH. These readouts connect the observed metabolite pattern with cellular energy status and biochemical regulation. Because measurements are noninvasive, researchers can investigate these properties without disrupting the sample, preserving the biological context of the observation.
Phosphorus-31 MRS supports research across muscle physiology, brain function, cancer metabolism, and metabolic disease. Its value differs by context: muscle studies can examine tissue energy metabolism, brain studies can investigate neural tissue biochemistry, and disease-oriented research can track altered metabolic or membrane-related states. The same noninvasive approach therefore applies across multiple biological systems.
Longitudinal monitoring allows biochemical changes to be followed over time in the same living system. Repeated measurements can reveal evolving patterns in tissue bioenergetics, mitochondrial function, intracellular pH, or membrane turnover rather than providing only a single time point. This capability is especially useful for studying progression, response, or changing metabolic states in biological research.