Pollen Microbiome
Pollen Microbiome - Related Videos
06:34
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
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Cited by 4 •
2017
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
01:22
Microbiome of the Eye
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2026
The human eye has a specialized microbiota that reflects its unique anatomical and immunological environment. This low-biomass microbial community predominantly colonizes the conjunctiva and eyelid margins, playing a vital role in ocular surface homeostasis and defense. Despite its proximity to the richly colonized facial skin, the ocular surface maintains a distinct microbial profile due to continuous mechanical and biochemical defense mechanisms.The conjunctival surface hosts fewer microbial...
10:51
Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device
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Cited by 68 •
2016
We describe an in vitro protocol to co-culture gut microbiome and intestinal villi for an extended period using a human gut-on-a-chip microphysiological system.
08:11
Collection and Identification of Pollen from Honey Bee Colonies
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Cited by 8 •
2021
We describe methods for collecting corbicular pollen from honey bees as well as protocols for color sorting, acetolysis, and microscope slide preparation of pollen for taxonomic identification. In addition, we present pellet color and taxonomic diversity of corbicular pollen collected from five cropping systems using pollen traps.
08:38
Application of Flow Vermimetry for Quantification and Analysis of the Caenorhabditis elegans Gut Microbiome
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2023
Caenorhabditis elegans is a powerful model to examine the molecular determinants driving host-microbiome interactions. We present a high throughput pipeline profiling the single animal levels of gut microbiome colonization together with key aspects of the C. elegans physiology.
FAQs
Pollen-associated microorganisms can originate from floral tissues, air, soil, and pollinators, so exposure to different sources shapes which organisms reach or enter the grains. Plant species and environmental conditions also contribute to variation in community composition. This variability helps explain why pollen microbiomes may differ across plants, locations, or biological interactions.
Microbial interactions can affect several stages of pollen function, including grain viability, germination, and pollen-tube growth. They may also change the chemical composition of pollen. Because these traits determine whether pollen remains functional and progresses toward fertilization, microbial effects provide a biological connection between microscopic communities and plant reproductive success.
Differences in microbial composition can be relevant because associated communities may alter how pollen performs after it is produced and transferred. Changes in viability, germination, or pollen-tube growth could influence the effectiveness of reproduction. Comparing plant species and environments therefore helps biologists examine how microbial variation contributes to differences in reproductive outcomes.
Researchers use both sequencing and culture-based methods to study microorganisms associated with pollen. Sequencing helps reveal community composition, including organisms that may not be recovered readily in culture, while culture-based approaches examine organisms that can be grown and studied directly. Using these complementary approaches provides a broader view of microbial diversity and pollen-associated interactions.
This research can clarify how plants, microorganisms, and pollinators affect one another. Because pollinators can contribute to microbial acquisition, they may influence the communities encountered by pollen during transfer. Linking those communities with pollen viability, germination, and pollen-tube growth helps biologists investigate connections between pollination biology, microbial ecology, and plant fertility.
Pollen microbiome studies have applications in understanding plant fertility and crop production, while also informing research on disease transmission and pollen-related allergies. These areas reflect different consequences of pollen-associated microorganisms: effects on reproductive performance, movement of microbial partners or agents, and interactions with pollen encountered by organisms. The same community can therefore matter across agricultural, ecological, and health-related research.