Pollen Tube Growth
Pollen Tube Growth - Related Videos
06:45
Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method
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Cited by 4 •
2023
Here, we describe an improvement of the semi-in vitro (SIV) method for observing pollen tube guidance and reception in Arabidopsis thaliana, which increases the receptivity of ovules. The high-throughput SIV cum septum method may be coupled with gametophyte marker lines and genetically encoded biosensors to monitor the dynamic process of fertilization.
06:34
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
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Cited by 4 •
2017
This work describes a protocol that uses a component-resolved approach to study sensitization to grass pollen allergens in a cohort of patients from southern China with allergic rhinitis and/or asthma.
10:56
Tube Thoracostomy
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2023
Source: Rachel Liu, BAO, MBBCh, Emergency Medicine, Yale School of Medicine, New Haven, Connecticut, USA Tube thoracostomy (chest tube placement) is a procedure during which a hollow tube is inserted into the thoracic cavity for drainage of fluid or air. Emergency chest tube insertion is performed for definitive treatment of tension pneumothorax, traumatic hemothorax, large-volume pleural effusions, and empyemas. Irrespective of the cause of air and fluid accumulation in the pleural space, the...
08:11
Collection and Identification of Pollen from Honey Bee Colonies
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Cited by 8 •
2021
We describe methods for collecting corbicular pollen from honey bees as well as protocols for color sorting, acetolysis, and microscope slide preparation of pollen for taxonomic identification. In addition, we present pellet color and taxonomic diversity of corbicular pollen collected from five cropping systems using pollen traps.
08:46
Strategic Endothelial Cell Tube Formation Assay: Comparing Extracellular Matrix and Growth Factor Reduced Extracellular Matrix
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Cited by 19 •
2016
This manuscript describes a tube formation assay to quantify the effects of a given compound or condition on angiogenesis by using endothelial cell tube formation in a controlled environment.
FAQs
Calcium gradients are concentrated at the growing apex, where they help coordinate the localized activity required for extension. Their tight regulation links cellular signaling with tip-focused secretion, allowing the tube to maintain a polarized growth pattern rather than expanding uniformly. This relationship makes pollen tubes useful for examining how calcium signals control directional cell growth.
Actin-guided vesicle trafficking directs secretory vesicles toward the growing tip, where tip-focused secretion supplies material for extension. This arrangement connects the cytoskeleton with membrane trafficking and preserves cell polarity. Because these processes are spatially organized, pollen tube growth provides a tractable system for studying how intracellular transport supports rapid, localized cellular expansion.
Growth begins only after compatible pollen germinates on the stigma, establishing a biological checkpoint before tube extension proceeds. As the tube advances, chemical and tissue cues guide it toward the ovary. These interactions connect pollen tube behavior with cell-cell signaling and help determine whether the tube reaches the reproductive target needed for successful fertilization.
A typical analysis follows compatible pollen from germination on the stigma through polarized tube elongation and guidance toward the ovary. Researchers can then relate the tube’s progress to tip-focused secretion, actin-guided trafficking, calcium regulation, and signaling from surrounding tissues. This sequence connects visible reproductive progression with the cellular mechanisms that support sperm delivery.
Pollen tubes provide a tractable model because their polarized extension makes cell polarity, cytoskeletal dynamics, membrane trafficking, and cell-cell signaling especially accessible for study. The same process also has a clear reproductive outcome: delivering sperm cells to the ovule. Consequently, experiments can connect fundamental cellular mechanisms with a biologically important event in flowering plants.
Research on pollen tube growth can inform plant breeding and fertility studies by clarifying how pollen germination, tube guidance, and sperm delivery contribute to reproductive success. It also helps investigate reproductive barriers that prevent compatible progression through plant tissues. These insights are relevant to understanding, and potentially addressing, factors associated with crop productivity.