Poultry Pathogen Analysis

Poultry pathogen analysis is the systematic detection and identification of infectious agents in chickens, turkeys, and other domestic birds, supporting disease prevention, flock health, and food safety. The process typically combines specimen collection from birds or their environment with culture-based testing, microscopy, immunoassays, or molecular methods such as polymerase chain reaction, which amplifies pathogen-specific genetic material for detection. In biology and veterinary research, these analyses help distinguish bacterial, viral, and parasitic infections, track transmission within flocks, and evaluate treatment or vaccination strategies. Results guide biosecurity measures, outbreak response, and surveillance programs that reduce production losses and limit the spread of zoonotic pathogens.

Poultry Pathogen Analysis - Related Videos

Research

JoVE EoE - Bacterial Pathogenesis and Host Interactions

Determining the Minimum Inhibitory Concentration of Enrofloxacin Against Avian Pathogenic E. coli

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2026

Begin with a suspension of avian pathogenic E. coli or APEC in growth media.APEC is a Gram-negative bacterium that infects poultry by entering through the respiratory tract.After breaching the mucosal layer, APEC adhere to epithelial cells, multiply locally, and disrupt the epithelial barrier to enter the bloodstream. The bacteria then spread to multiple organs, causing systemic infection.Add an equal volume of the bacterial suspension to each well of a multi-well plate containing increasing...

Detection of Fish Pathogens Using Variable Number Tandem Repeat (VNTR) Analysis

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2025

Source: Gulla, S., et al. Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis. J. Vis. Exp. (2019).This video demonstrates the procedure for separating fluorescently labeled variable number tandem repeat (VNTR) amplicons, derived from a fish-pathogenic bacterium, using capillary electrophoresis to generate size-specific electropherogram profiles for bacterial identification.

Single-Cell Analysis of Gene Expression from Plant-Extracted Pathogens

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2026

Source: Rufián, J. S., et al. Single-Cell Analysis of the Expression of Pseudomonas syringae Genes within the Plant Tissue. J. Vis. Exp. (2022).This video demonstrates a method for analyzing gene expression in plant-extracted pathogenic bacteria at the single-cell level using a fluorescently tagged transcription factor. Confocal imaging reveals heterogeneity in virulence gene expression, highlighting functional diversity within a clonal bacterial population.

Analysis of Macrophage Receptor Interactions with Pathogenic Bacteria Using Flow Cytometry

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2026

Source: Barbero, A. M., et al. Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1. J. Vis. Exp. (2025)This video demonstrates the procedure for labeling host immune receptors that bind bacterial surface antigens, enabling their detection through flow cytometry.

Research

JoVE Journal - Immunology and Infection
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Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

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Cited by 22 •

2013

We describe a method for tracking the endomembrane rupture elicited by the intracellular bacteria Shigella flexneri and Mycobacterium tuberculosis upon host cell invasion. Our assay makes use of CCF4, a host cytoplasmic FRET probe in live or fixed cells. This reporter is degraded by an enzyme activity present on the bacterial surface.

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