Rapid Tissue Sampling
Rapid Tissue Sampling - Related Videos
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
08:48
Quantitation of γH2AX Foci in Tissue Samples
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Cited by 7 •
2010
Quantitation of DNA double-strand breaks on the basis of γH2AX foci has become an invaluable tool, particularly in radiation biology, for the evaluation of tissue radiosensitivity and effects of radiation modifying compounds. Here we demonstrate the use of an immunofluorescence assay for quantitation of γH2AX foci in tissue samples.
12:39
Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit
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Cited by 4 •
2008
The complete genotyping of a mouse tail sample, including tissue digestion and PCR readout, is done in one and a half hours using Sigma's SYBR Green Extract-N-Amp Tissue PCR kit.
02:08
A Hydrophobic Tissue Clearing Method for a Rat Brain Tissue Sample
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2025
Source: Kirchner, K.N., et al., A Hydrophobic Tissue Clearing Method for Rat Brain Tissue. J. Vis. Exp. (2020).This video demonstrates the method of hydrophobic tissue clearing of a rat brain tissue sample. The sample is sequentially dehydrated in methanol, treated with a dichloromethane and methanol mixture to dissolve lipids and pigments, and transferred to dibenzyl ether for complete tissue clearing, enabling detailed imaging without thin sectioning.
03:30
Snap Freezing of Muscle Tissue for Sectioning: A Protocol to Obtain Ultrathin Sections of Rapidly Frozen Murine Muscle Tissue
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2023
This video describes the procedure for snap freezing muscle tissue and obtaining its thin sections. The technique can help study cancer-related cachexia in colon cancer mouse models.
FAQs
A short interval helps preserve more than convenience: tissue can undergo degradation or stress-related changes while it remains untreated. Those changes may alter cellular structure, molecular composition, or the biological state being measured. Rapid handling therefore improves the chance that microscopy, histology, or molecular analysis reflects the specimen at collection rather than a later altered condition.
The sample must be handled and preserved in conditions that limit degradation and unintended biological change. If those conditions are inappropriate or applied inconsistently, specimens may differ because of processing-related effects rather than true tissue differences. Standardizing this part of the workflow supports more reliable comparisons across samples and helps maintain the features required for downstream biological analysis.
Representativeness depends on whether the collected piece retains the cellular and molecular features relevant to the intended comparison, not simply on its size. A small biopsy or excised sample can support analysis when collection and stabilization are consistent. This approach helps researchers compare healthy and affected tissues with less variation introduced by handling differences.
Delayed collection can allow stress or degradation to change the specimen before stabilization. Rapid sampling reduces that interval, making observed differences more likely to reflect biological differences between tissues rather than changes introduced after removal. This distinction is especially important when comparing healthy and affected samples or examining biological changes that may occur over short periods.
A practical workflow begins with obtaining a small biopsy or excised specimen, followed by prompt transfer into the selected stabilization or preservation condition. The sample is then handled in a way that maintains its cellular structure and molecular composition until analysis. Coordinating these stages reduces delay and supports consistent preparation for histology, microscopy, or molecular profiling.
The features preserved during collection determine which biological questions can be examined. Histology and microscopy can use retained cellular structure, while molecular profiling depends on maintaining molecular composition. Studies of tissue physiology also require attention to the biological state. Rapid sampling provides a common collection approach for these investigations when handling protects the relevant features.
Rapid Tissue Sampling is particularly valuable when investigators need to study tissue physiology, disease-related differences, or biological changes that may be altered by time outside the organism. It supports comparisons between healthy and affected tissues and helps produce consistent specimens for time-sensitive investigations. The resulting samples can contribute to histological, microscopic, or molecular studies.