Rat Blood Collection
Rat Blood Collection - Related Videos
05:12
Repeated Blood Collection from Tail Vein of Non-Anesthetized Rats with a Vacuum Blood Collection System
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Cited by 25 •
2017
Here, we describe a simple tail vein blood sampling method in non-anesthetized rats using a vacuum extraction tube system. This method reduces the risk of direct exposure to blood and simplifies taking multiple samples from a single venipuncture.
01:30
Blood Collection in Rabbits
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Blood sampling is commonly performed in laboratory rabbits for research and clinical applications. The collection site is selected based on the required sample volume, sampling frequency, and study objectives. As a general...
01:33
Blood Collection in a Pig Model
0 Views •
2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Blood collection is a common procedure in swine research and clinical studies. The choice of sampling site depends on the animal's age, body size, the volume of blood required, the study objectives and approved IACUC...
06:17
Repeated Blood Collection for Blood Tests in Adult Zebrafish
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Cited by 87 •
2015
Repeated blood sampling is necessary and important in animal research. We developed a novel, non-lethal and reliable method for repeated blood collection from adult zebrafish, and applied this method to the study of blood biochemistry, including glucose metabolism.
03:11
Blood Collection for Biochemical Analysis in Adult Zebrafish
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Cited by 111 •
2012
This paper presents a technique for collection of blood from the dorsal aorta of Zebrafish. It also provides instructions for obtaining serum for use in biochemical analyses, such as tests for determining cholesterol and triglyceride levels.
FAQs
Site selection influences access to a blood vessel, the likelihood of tissue injury, and the risk of contamination. An appropriate site allows the sample to be obtained efficiently while limiting unnecessary manipulation of the animal. Consistent selection also helps reduce variation between samples, supporting more reliable hematology, clinical chemistry, pharmacology, and biomedical measurements.
Proper restraint stabilizes the rat during vessel access and helps the operator use a needle or lancet accurately. This can reduce sudden movement, tissue injury, stress, and accidental contamination. Because sampling conditions can influence sample quality, restraint should support both safe handling and controlled collection rather than relying on forceful or prolonged manipulation.
The collection tube determines the type of specimen available for analysis. Tubes containing anticoagulants preserve blood without clotting, allowing collection of whole blood or preparation of plasma. Tubes without anticoagulants allow serum to be obtained. Selecting the appropriate tube is therefore essential because hematology, clinical chemistry, and pharmacology assays may require different blood products.
Tissue injury, animal stress, contamination, and poor handling or processing can all reduce sample quality. These problems may arise during site access, restraint, transfer into collection tubes, or later preparation. Controlling each stage helps preserve the sample and improves the reliability of measurements used to evaluate biological or treatment-related responses.
A controlled workflow begins by choosing a suitable collection site and preparing the rat for safe restraint. A sterile needle or lancet is then used to access the vessel, and the blood is transferred into a tube selected for whole blood, plasma, or serum. Careful handling and processing follow to preserve sample quality and limit contamination.
The choice depends on the measurement and the required sample form. Whole blood is retained when the blood itself must be examined, whereas plasma or serum is prepared when the analysis requires a separated liquid component. Using the correct tube and processing approach helps align the specimen with hematology, clinical chemistry, pharmacology, or other biomedical research goals.
Blood samples can provide material for measurements in hematology, clinical chemistry, pharmacology, and biomedical research, linking an animal’s physiology with experimental questions. At the same time, the procedure must minimize tissue injury, stress, and contamination. Balancing analytical needs with controlled handling supports both interpretable results and responsible use of rats in biological studies.