Recalcitrant Bacteria
Recalcitrant Bacteria - Related Videos
11:07
In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)
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2025
This protocol describes the design and use of an isolation chip (iChip) for in situ cultivation of recalcitrant soil microbes in their natural environment. It also describes the in situ cultivation of antimicrobial-producing soil bacteria. The protocol can be adapted to recover bacteria from diverse niches with other desirable properties.
09:38
Hybrid-Cut: An Improved Sectioning Method for Recalcitrant Plant Tissue Samples
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Cited by 22 •
2016
This protocol describes a simple Hybrid-Cut tissue sectioning method that is useful for recalcitrant plant tissues. Good quality tissue sections enable anatomical studies and other biological studies including in situ hybridization (ISH).
11:31
High-throughput Screening of Recalcitrance Variations in Lignocellulosic Biomass: Total Lignin, Lignin Monomers, and Enzymatic Sugar Release
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Cited by 19 •
2015
Plant cell wall structure and chemistry traits are evaluated to identify ideal feedstocks for biofuels and bio-materials. Standard methods have limitations when applied to large data sets. These high-throughput pretreatment, enzyme saccharification, and pyrolysis-molecular beam mass spectrometry methods compare large numbers of biomass samples with decreased experimental time and cost.
10:57
Culturing and Enumerating Bacteria from Soil Samples
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Authors: Bradley Schmitz and Luisa Ikner Surface soils are a heterogeneous mixture of inorganic and organic particles that combine together to form secondary aggregates. Within and between the aggregates are voids or pores that visually contain both air and water. These conditions create an ideal ecosystem for bacteria, so all soils contain vast populations of bacteria, usually over 1 million...
13:20
Detection of Bacteria Using Fluorogenic DNAzymes
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Cited by 17 •
2012
We have recently reported a novel approach for generating fluorogenic DNAzyme probes that can be applied to set up a simple, "mix-and-read" fluorescent assay for bacterial detection. These special DNA probes catalyze the cleavage of a chromophore-modified DNA-RNA chimeric substrate in the presence of crude extracellular mixture (CEM) produced by a specific bacterium, thereby translating bacterial detection into fluorescence signal generation. In this report we will describe key experimental...
FAQs
Fastidious nutritional requirements can make a bacterium dependent on nutrients or combinations that routine laboratory media do not provide. If those needs are absent, the organism may fail to grow even when viable, leading investigators to mistake cultivation failure for biological absence. Specialized media are therefore used to better match the organism’s nutritional demands and improve recovery for further study.
Several physiological conditions can block recovery at once. Sensitivity to oxygen or temperature means routine incubation may create an unsuitable environment, while slow growth can leave colonies undetected during standard observation periods. Dormancy adds another barrier because cells may remain inactive rather than visibly proliferating. These factors explain why changing cultivation conditions can be more informative than repeating the same protocol.
Some Recalcitrant Bacteria may depend on host cells or interactions within microbial communities. Isolation in a simple, cell-free culture can therefore remove conditions required for survival or growth, even if the organism thrives in its natural setting. Co-culture and enrichment approaches address this problem by retaining or selecting supportive biological relationships rather than assuming the bacterium grows independently.
An effective strategy begins by matching the suspected barrier to the method. Specialized media address nutritional limitations; co-culture can preserve relationships with host cells or other microbes; enrichment can favor the target within a mixed population; and molecular approaches can provide information when growth remains inaccessible. Using these options helps distinguish true absence from failure of the chosen cultivation conditions.
Molecular approaches can help characterize organisms that remain uncultured and connect them with ecosystem function, disease, or other biological roles. This makes molecular analysis valuable not only as a substitute for routine growth, but also as a way to investigate hidden microbial diversity and identify organisms that conventional isolation would miss.
Characterizing these organisms can reveal hidden microbial diversity, clarify how uncultured microbes contribute to ecosystem function, and support environmental and clinical diagnostics. It may also expose previously inaccessible metabolic pathways and antimicrobial targets, giving biology a broader view of microbial processes that standard cultivation alone cannot capture.