Reconstructed Human Epidermis
Reconstructed Human Epidermis - Related Videos
08:49
Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
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Cited by 9 •
2021
This protocol describes a straightforward method to cultivate three-dimensional reconstituted human epidermis in a reproducible and robust manner. Additionally, it characterizes the structure-function relationship of the epidermal barrier model. The biological responses of the reconstituted human epidermis upon proinflammatory stimuli are also presented.
11:02
The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal Skin and Melanoma Progression
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Cited by 111 •
2011
In this report, we describe the three-dimensional skin reconstruct model which mimics human skin in architecture and composition. Melanocyte physiology, melanoma progression and the fate of dermal stem cells have been investigated using the skin reconstruct model. The model is also useful as a preclinical tool for drug assessment.
04:21
Organotypic 3D Skin Culture: A Cassette-based In Vitro Culture Technique to Regenerate Human Epidermis on Acellular Dermis
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2023
This video protocol describes a cassette-based in vitro culture technique to generate human organotypic skin using epidermal keratinocytes and devitalized human dermis. They mimic the function and physiology of their in vivo tissue counterparts.
01:24
Cells of the Epidermis
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2023
The epidermis is made of four or five layers of epithelial cells, depending on its location in the body. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, stratum lucidum, and stratum corneum. The cells in all these layers except the stratum basale are called keratinocytes, a type of cell that manufactures and stores the protein keratin. The keratinocytes in the stratum corneum are dead and regularly slough away, being replaced by cells from...
21:16
An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
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Cited by 57 •
2009
In this video, we demonstrate the EpiDerm Skin Irritation test (EpiDerm SIT) developed and validated for in vitro skin irritation testing of chemicals, including cosmetic and pharmaceutical ingredients.
FAQs
Human keratinocytes form the cellular basis of the tissue, while the permeable support provides a culture surface through which the developing cells can be maintained. Exposing the culture to an air-liquid interface then encourages proliferation and differentiation. Together, these conditions allow the cells to organize into stratified layers and develop a surface barrier suitable for biological investigation.
The air-liquid interface supplies a developmental condition that promotes progression beyond simple cell growth. Under this exposure, keratinocytes proliferate, differentiate, and organize into multiple layers, producing structural features associated with the epidermal surface. This matters because studies of barrier function, development, and disease-related changes require a tissue with organized layers rather than an undifferentiated cell population.
Researchers can examine epidermal development, formation of stratified layers, surface barrier function, and responses associated with wounding or disease-related change. The model also supports investigation of cellular mechanisms within the epidermis. These features provide several complementary ways to study how epidermal structure and function change under controlled laboratory conditions.
Reconstructed Human Epidermis provides a controlled laboratory platform in which epidermal processes can be examined without relying exclusively on native human tissue. Its defined culture setting supports focused investigation of cellular mechanisms, barrier behavior, and responses to test substances. It therefore complements native tissue studies by offering a reproducible experimental context for comparing biological effects.
Production begins by culturing human keratinocytes on a permeable support. The developing culture is then maintained with exposure to an air-liquid interface, which promotes proliferation and differentiation. As the cells organize into stratified layers and form a surface barrier, the resulting tissue can be used for studies of epidermal biology, responses to topical substances, or disease-related changes.
Researchers can apply this model when they need a controlled platform for examining topical formulations or chemical irritation. The organized tissue and surface barrier provide a biologically relevant setting for observing how test substances affect epidermal structure and function. Such experiments can support comparison of responses while reducing dependence on native human tissue.
In wound research, the model supports examination of epidermal responses after injury-related challenges. In disease-focused studies, researchers can investigate changes in tissue structure, barrier function, and underlying cellular mechanisms associated with disease-related conditions. Because the culture environment is controlled, the system helps connect observed tissue-level changes with specific biological processes in the epidermis.