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The Three-Dimensional Human Skin Reconstruct Model: a Tool to Study Normal Skin and Melanoma Progression
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Chapters
- 00:05Title
- 01:24Dermal Layer Preparation
- 03:22Generation of the Epidermis
- 05:21Day 18 Skin Reconstruct Harvesting and Sectioning
- 07:01Graft a Skin Reconsruct on a Mouse
- 08:42Representative Skin Reconstructs of Normal Melanocytes, Dermal Stem Cells and Melanoma Tumors
- 10:43Conclusion
In this report, we describe the three-dimensional skin reconstruct model which mimics human skin in architecture and composition. Melanocyte physiology, melanoma progression and the fate of dermal stem cells have been investigated using the skin reconstruct model. The model is also useful as a preclinical tool for drug assessment.
Tags
Three-dimensional Human Skin Reconstruct ModelIn Vitro StudiesExperimental Skin Biology2-dimensional Monocultures3D Extra-cellular MatrixCell-cell InteractionsMouse ModelsCellular ArchitecturePhysiologyMelanocytesHair FolliclesBasal Layer Of The Epidermis3D Human Skin Reconstruct ModelsDermisFibroblastsCollagen I MatrixEpidermisStratified Differentiated KeratinocytesFunctional Basement MembraneScaffoldingNutrient DeliveryCell-to-cell InteractionMelanocyte HomeostasisMelanoma Progression
