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JoVE Journal
Bioengineering

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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
 

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Article DOI: 10.3791/61802
May 28th, 2021

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Summary May 28th, 2021

This protocol describes a straightforward method to cultivate three-dimensional reconstituted human epidermis in a reproducible and robust manner. Additionally, it characterizes the structure-function relationship of the epidermal barrier model. The biological responses of the reconstituted human epidermis upon proinflammatory stimuli are also presented.

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