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JoVE Journal
Bioengineering

This content is Open Access.

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale
 

Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

Article DOI: 10.3791/61802-v 08:49 min May 28th, 2021
May 28th, 2021

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Summary

This protocol describes a straightforward method to cultivate three-dimensional reconstituted human epidermis in a reproducible and robust manner. Additionally, it characterizes the structure-function relationship of the epidermal barrier model. The biological responses of the reconstituted human epidermis upon proinflammatory stimuli are also presented.

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Three-dimensional Reconstructed Human Epidermis In Vitro Model Alternative Testing Methods Cosmetic Ingredient Testing Tissue Suppliers Laboratory Reconstitution Culturing Process Culture Medium Composition Pro-inflammatory Triggers Oxidative Stress Triggers Gene Silencing Biological Process Stimulation Cryo-preserved Normal Human Epidermal Keratinocytes (NHEKs) Water Bath Incubation Cell Suspension Transfer
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