Rfp Detection
Rfp Detection - Related Videos
06:07
A Novel RFP Reporter to Aid in the Visualization of the Eye Imaginal Disc in Drosophila
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Cited by 1 •
2009
We describe a novel red fluorescent protein (RFP) reporter that is expressed specifically in the Drosophila eye. We detail a methodology for dissection of the eye imaginal disc and how this reporter can be used to aid in the dissection and identification of specific cell types in the developing eye.
03:33
Fluorescence-Based Detection and Purification of Recombinant Vaccinia Virus
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2026
Source: Yuan, M., et al. A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors. J. Vis. Exp. (2016)This video demonstrates the process of performing a fluorescent plaque assay using recombinant vaccinia virus encoding red fluorescent protein (RFP) in fibroblast cells, followed by plaque isolation and purification for further analysis.
02:02
Detection of a Target Analyte Using a Bacterial Biosensor
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2025
Source: Barber, A. E., et al. Preparation and Application of a New Bacterial Biosensor for the Presumptive Detection of Gunshot Residue. J. Vis. Exp. (2019).This video demonstrates the detection of specific analytes using a bacterial biosensor. A bacterial culture carrying a plasmid with a red fluorescent protein (RFP) gene under an analyte-sensitive promoter is taken. After thoroughly wiping the test surface, a small section of the wipe is immersed in the culture and incubated. The analyte...
02:40
Detecting SMAD Pathway Activation Using Dual Adenovirus Transduction
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2026
Source: Chen, H., et al. Live Cell Imaging of the TGF-β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System. J. Vis. Exp. (2018).This video demonstrates a method to study SMAD pathway activation in breast cancer cells using dual adenoviral infection. One virus delivers a constitutively expressed GFP gene, while the other carries an RFP reporter under a SMAD-sensitive promoter. Upon TGF-beta stimulation, SMAD proteins are activated, forming a complex that induces RFP...
09:10
Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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Cited by 17 •
2010
This protocol describes a simple adhesive-tape-based approach for sampling of tomato and other fresh produce surfaces, followed by rapid whole cell detection of Salmonella using fluorescence in situ hybridization (FISH).
FAQs
The key optical distinction is that RFP absorbs excitation light and emits light at a longer wavelength. This separation allows a detector or microscope to distinguish the reporter signal from the illumination used to activate it. Without separating these wavelengths, the recorded signal would be harder to attribute specifically to RFP.
Optical filters and spectral detection provide different ways to isolate the red signal from background. Filters selectively limit which wavelengths reach the detector, whereas spectral detection distinguishes signal according to its wavelength characteristics. The choice affects how clearly researchers can identify RFP fluorescence in images or measurements.
Qualitative analysis focuses on whether fluorescence appears and where it is located, while quantitative analysis measures the detected signal for numerical comparison. The first approach can reveal expression patterns or protein distribution; the second can support assessment of changes in signal. Both rely on distinguishing fluorescence from background.
A basic workflow starts with illumination at an appropriate excitation wavelength, followed by collection of the emitted red fluorescence. An instrument or fluorescence microscope then separates the signal from background with optical filters or spectral detection. Researchers can record the result qualitatively or use the measured signal for quantitative analysis.
In biology, RFP detection can connect a fluorescent signal to several kinds of observation: transgene expression, protein localization, cellular activity, labeled-cell distribution, and biological changes over time. These uses let researchers examine where a reporter-associated event occurs and whether its observable signal changes, supporting studies in cell and molecular biology.
The method is relevant across cell biology, developmental studies, molecular biology, and biomedical research because the same optical readout can be used to visualize distribution, monitor expression, or assess change. Its flexibility supports both image-based observations and numerical measurements, allowing researchers to choose an outcome that matches the biological question.