Rna Primers
Rna Primers - Related Videos
11:14
Primer-Free Aptamer Selection Using A Random DNA Library
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Cited by 20 •
2010
SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere with the selection process (false positives and negatives). Here we present a primer-free protocol.
09:06
RNA Interference in Ticks
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Cited by 49 •
2011
A method for RNA interference (RNAi) by injection of dsRNA into unfed ticks is described. RNAi is the most widely used gene-silencing technique in ticks where the use of other methods of genetic manipulation has been limited.
15:28
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
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Cited by 41 •
2009
We present a method of targeted ancient DNA sequence retrieval, which we used to reconstruct the complete mitochondrial genomes of five Neandertal individuals. Comparison of these sequences with present day humans suggests that Neandertals had a long term low effective population size.
09:54
RNA-Seq
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2023
Among different methods to evaluate gene expression, the high-throughput sequencing of RNA, or RNA-seq. is particularly attractive, as it can be performed and analyzed without relying on prior available genomic information. During RNA-seq, RNA isolated from samples of interest is used to generate a DNA library, which is then amplified and sequenced. Ultimately, RNA-seq can determine which genes are expressed, the levels of their expression, and the presence of any previously unknown transcripts.
10:17
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
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Cited by 7 •
2017
Most common gut content analyses of macroinvertebrates are visual. Requiring intense knowledge about morphological diversity of prey organisms, they miss soft bodied prey and, due to strong comminution of prey, are nearly impossible for some organisms, including amphipods. We provide detailed, novel genetic approaches for macroinvertebrate prey identification in the diet of amphipods.
FAQs
Primase first makes a short RNA sequence that is complementary to the DNA template. This complementary pairing positions the primer at the intended starting region and leaves a free 3′-hydroxyl group. DNA polymerase uses that existing end to begin adding deoxyribonucleotides, creating a controlled handoff between the enzymes.
The leading strand can be extended from an initial primer as synthesis follows the template continuously, whereas the lagging strand is produced in separate Okazaki fragments. Each fragment needs its own starting point, so repeated priming supports discontinuous synthesis. Comparing these patterns clarifies how both strands are copied within one replication process.
Primer processing occurs after DNA synthesis has progressed: cellular enzymes remove the RNA segment, replace it with DNA, and seal the remaining nicks. This sequence converts a temporary RNA-containing start into continuous DNA. Its outcome is chromosome continuity, because gaps or unsealed breaks would leave the replicated molecule structurally incomplete.
By focusing on primase-created initiation, polymerase extension, and primer removal or replacement, researchers can examine which stage an inhibitor affects. This framework links altered DNA synthesis to particular replication events rather than treating replication as a single process. It therefore supports more precise study of how replication inhibitors influence genome copying.
They provide a framework for studying how DNA replication begins, how leading and lagging strands are coordinated, and how temporary RNA segments are processed into continuous DNA. These questions connect primer behavior with genome copying, chromosome continuity, and mutation risk, making the topic useful across genetics and molecular biology.
In biotechnology, primer biology supplies a conceptual basis for examining DNA synthesis, replication initiation, and the processing steps that restore continuous DNA. Researchers can use this framework when interpreting replication-related experiments or considering how replication inhibitors affect genome copying. Its value comes from linking molecular events to DNA replication.