Rna Removal
Rna Removal - Related Videos
09:54
RNA-Seq
0 Views •
2023
Among different methods to evaluate gene expression, the high-throughput sequencing of RNA, or RNA-seq. is particularly attractive, as it can be performed and analyzed without relying on prior available genomic information. During RNA-seq, RNA isolated from samples of interest is used to generate a DNA library, which is then amplified and sequenced. Ultimately, RNA-seq can determine which genes are expressed, the levels of their expression, and the presence of any previously unknown transcripts.
01:53
RNA Stability
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2019
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
01:32
RNA Splicing
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2019
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
11:32
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Cited by 20 •
2017
Here, we detail the method of Sequencing of Psoralen crosslinked, Ligated, and Selected Hybrids (SPLASH), which enables genome-wide mapping of intramolecular and intermolecular RNA-RNA interactions in vivo. SPLASH can be applied to study RNA interactomes of organisms including yeast, bacteria and humans.
02:23
RNA Editing
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2020
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
FAQs
RNases remove RNA by hydrolyzing the phosphodiester bonds that connect nucleotides in RNA molecules. This breaks intact RNA into smaller degraded fragments rather than leaving it as a continuous nucleic acid population. A subsequent purification step helps separate those fragments from DNA and other sample components, producing material better suited to downstream molecular biology analyses.
Residual RNA contributes unwanted nucleic acid content to a preparation, which can affect how much material is measured as DNA. It may also interfere with PCR, genotyping, cloning, or sequencing workflows and complicate interpretation of the resulting data. Removing this contamination improves sample quality and supports more accurate, reproducible conclusions from DNA-focused experiments.
Purification alone separates components, whereas RNA removal typically adds an enzymatic step that specifically breaks down the RNA population before separation. The combined strategy addresses both the chemical degradation of RNA and the physical removal of its fragments. This distinction matters when the desired material is DNA and remaining RNA could distort measurements or downstream reactions.
The extent of removal determines how confidently researchers can attribute measured nucleic acid content and downstream signals to DNA rather than mixed nucleic acids. Incomplete removal can leave contamination that affects quantification or experimental reactions, while effective treatment followed by purification yields cleaner material. Consequently, RNA control contributes directly to reproducibility and reliable interpretation.
A typical workflow begins by treating the biological sample, nucleic acid preparation, or reaction mixture with ribonucleases. These enzymes hydrolyze RNA into smaller fragments. The treated material then undergoes purification to separate the degraded RNA from DNA, proteins, and other components. The resulting preparation can be directed to a DNA-centered downstream analysis.
The central components are the material containing unwanted RNA, a ribonuclease treatment, and a purification step. The enzyme supplies the chemical activity needed to break RNA bonds, while purification separates the resulting fragments from retained DNA, proteins, or other constituents. Together, these components determine whether the final preparation is sufficiently clean for its intended analysis.
Researchers use RNA removal when RNA contamination could compromise DNA quantification, PCR, genotyping, cloning, or sequencing. It is particularly relevant whenever a preparation or reaction mixture contains both nucleic acid types but the experiment requires cleaner DNA-focused material. Controlling RNA content helps improve sample quality, experimental reproducibility, and the reliability of biological conclusions.