Soil Microorganisms

Soil microorganisms are microscopic life forms, including bacteria, fungi, archaea, and protozoa, that inhabit soil and drive essential biological processes. They break down organic matter by secreting enzymes, recycle nutrients such as carbon and nitrogen, and interact with plant roots through symbioses that can influence nutrient uptake and growth. These communities help form soil structure, regulate fertility, and contribute to ecosystem productivity. Studying soil microorganisms supports research in biology, agriculture, environmental monitoring, and bioremediation, while improving understanding of carbon storage, plant health, and how soil ecosystems respond to climate and land-use changes.

Soil Microorganisms - Related Videos

Education

JoVE Science Education - Environmental Sciences

Visualizing Soil Microorganisms via the Contact Slide Assay and Microscopy

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2023

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Soil comprises the thin layer at the earth’s surface, containing biotic and abiotic factors that contribute to life. The abiotic portion includes inorganic particles ranging in size and shape that determine the soil’s texture. The biotic portion incorporates plant residues, roots, organic matter, and microorganisms. Soil microbe abundance and diversity is expansive, as...

Research

JoVE Journal - Biology

Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture

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Cited by 25 •

2013

Plastic films labeled "biodegradable" are commercially available for agricultural use as mulches. Tillage represents an attractive disposal method, but degradation under field conditions is poorly understood. The purpose of this study was to develop methods for isolating native soil fungi and bacteria that colonize plastic mulch films after field burial.

Research

JoVE Journal - Biochemistry
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Isolation of Soil Microorganisms Using iChip Technology

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Cited by 2 •

2025

The iChip technique makes use of an inexpensive and simple in-situ isolation device that increases the rates of novel microorganism discovery from the soil. Novel microorganisms can be used for further study pertaining to the soil microbiome or natural product discovery, amongst other applications.

Quantifying Environmental Microorganisms and Viruses Using qPCR

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2023

Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Quantitative polymerase chain reaction (qPCR), also known as real-time PCR, is a widely-used molecular technique for enumerating microorganisms in the environment. Prior to this approach, quantifying microorganisms was limited largely to classical culture-based techniques. However, the culturing of microbes from environmental samples can be particularly challenging, and...

Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms

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Cited by 2 •

2022

This protocol presents a detailed summary of strategies to inoculate plant roots with soil-borne microbes. Exemplified for the fungi Verticillium longisporum and Verticillium dahliae, three different root infection systems are described. Potential applications and possible downstream analyses are highlighted, and advantages or disadvantages are discussed for each system.

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