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Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture
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Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture

Isolation of Native Soil Microorganisms with Potential for Breaking Down Biodegradable Plastic Mulch Films Used in Agriculture

DOI:
10.3791/50373-v

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13:38 min

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May 10, 2013

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, , , , , , ,
1Biology Department,Western Washington University, 2Washington State University Northwestern Research and Extension Center, 3Department of Plant and Soil Science,Texas Tech University

Chapters

  • 00:05Title
  • 01:28Preparation of Bioassay Materials: Surface Decontamination of Biodegradable Mulch Films
  • 02:48Plate Bioassay Setup
  • 03:41Isolation of Fungi
  • 06:20Initial Selection of Plastic-degrading Fungi
  • 07:58Stringent Confirmation of Plastic Utilization via Liquid Bioassay
  • 10:21Representative Biofilm Degradation Results
  • 13:07Conclusion

Summary

Automatic Translation

Automatic Translation

Plastic films labeled "biodegradable" are commercially available for agricultural use as mulches. Tillage represents an attractive disposal method, but degradation under field conditions is poorly understood. The purpose of this study was to develop methods for isolating native soil fungi and bacteria that colonize plastic mulch films after field burial.

Tags

Keywords: Soil MicroorganismsBiodegradable Plastic Mulch FilmsPlastic DegradationFungal IsolatesAgricultural SoilsPlastic FilmsCarbon SourceScanning Electron MicroscopyRibosomal RNA Gene SequencingBacterial Isolation

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