Sonication Process
Sonication Process - Related Videos
07:24
Sonication Extraction of Lipid Biomarkers from Sediment
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2023
Source: Laboratory of Jeff Salacup - University of Massachusetts Amherst The material comprising the living "organic" share of any ecosystem (leaves, fungi, bark, tissue; Figure 1) differs fundamentally from the material of the non-living "inorganic" share (rocks and their constituent minerals, oxygen, water, metals). Organic material contains carbon linked to a series of other carbon and hydrogen molecules (Figure 2), which distinguishes it from inorganic material. Carbon's wide valency range...
10:35
Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
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Cited by 16 •
2017
Differential diagnostics in painful arthroplasty is crucial for treatment success. Joint aspiration is routinely performed preoperatively. Multiplex polymerase chain reaction of the joint aspirate and the sonication fluid, a feasible tool for quick pathogen detection from these samples is described.
09:05
Generation and Quantitative Analysis of Pulsed Low Frequency Ultrasound to Determine the Sonic Sensitivity of Untreated and Treated Neoplastic Cells
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2015
Selective damage of human leukemia cells can be achieved through a novel approach of applying low frequency ultrasound both with and without chemotherapeutic pretreatment of leukemic and normal hematopoietic cells.
03:22
Low Frequency Ultrasound Application: A Method to Determine the Effect of Sonication on Human Leukemia Cells
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2023
This video describes the selective damage of human leukemia cells by using low frequency ultrasound. This application of low frequency ultrasound in a preclinical in vitro condition demonstrates that cell destruction can be attained in order to examine and compare the effects of sonication on leukemia and normal cells.
10:10
Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ
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Cited by 5 •
2014
Immunostaining is an effective technique for visualizing specific cell types and proteins within tissues. By utilizing sonication, the protocol described here alleviates the need to dissect Drosophila melanogaster tissues from late-stage embryos and larvae before immunostaining. We provide an efficient methodology for the immunostaining of formaldehyde-fixed whole mount larvae.
FAQs
Microscopic cavitation bubbles form within the liquid when pressure waves pass through the sample. Their rapid formation and collapse produce localized shear forces, which can break cell membranes and loosen material within suspensions. This physical disruption helps release intracellular contents for downstream processing, including nucleic-acid and protein extraction, without relying solely on chemical treatment.
These conditions determine how much mechanical disruption the sample receives. Greater power or longer treatment can increase membrane breakage and particle dispersion, while temperature and sample volume influence how the energy is distributed through the liquid. Researchers adjust the settings to obtain effective processing while limiting unwanted effects on sensitive biological molecules.
Localized shear concentrates mechanical stress at small regions of the sample, supporting membrane disruption, homogenization, and particle dispersion. This matters because biological material may need to be opened or evenly distributed before its contents can be recovered. The resulting preparation can improve access to intracellular components while maintaining control over the extent of physical disruption.
Effective processing requires enough sound energy to disrupt cells or mix the suspension, but not so much that sensitive contents are compromised. Controlling power, treatment duration, temperature, and sample volume provides this balance. The appropriate combination depends on whether the goal is strong lysis, general homogenization, extraction, or preparation of a stable biological suspension.
A basic workflow begins by placing the biological material in a liquid system and selecting either an ultrasonic probe or bath. The operator then controls power, duration, temperature, and sample volume while applying the treatment. Afterward, the processed material can be used as a disrupted sample, homogenate, suspension, or source for intracellular-content extraction.
Researchers may choose this technique when they need to break cell membranes, homogenize biological material, disperse particles, or prepare a suspension. It is also useful before extracting nucleic acids or proteins because mechanical disruption can release intracellular contents. The method therefore supports several preparation stages rather than serving only as a cell-lysis approach.
The treatment can produce several distinct preparation outcomes, depending on the controlled conditions. It may create a more uniform homogenate, disperse particles throughout a suspension, or release intracellular nucleic acids and proteins for extraction. These outcomes make sonication relevant to biological workflows that require either physical sample disruption or improved mixing before analysis.