Spongy Mesophyll

Spongy mesophyll is a layer of loosely arranged, living cells within a plant leaf that supports gas exchange and photosynthesis. Its irregular cells create interconnected air spaces beneath the compact palisade mesophyll, allowing carbon dioxide to diffuse inward, while oxygen and water vapor move toward the stomata and out of the leaf. Many spongy mesophyll cells contain chloroplasts, enabling them to capture light and produce sugars, although their primary role is efficient internal transport of gases. Studying this tissue helps explain leaf anatomy, photosynthetic performance, transpiration, and how plants respond to environmental conditions such as drought or limited carbon dioxide.

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Education

JoVE Core - Anatomy and Physiology

Spongy Bone

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2025

All bones comprise an outer layer of compact bone, and an interior made up of spongy bone tissue, also called cancellous or trabecular bone. In long bones, spongy bone tissue is mainly found in the interior of the epiphyses (broad ends of the bone). Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...

Research

JoVE Journal - Biology
Free Sample

Scalable Transfection of Maize Mesophyll Protoplasts

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Cited by 10 •

2023

Here, we present a high-throughput protocol to transiently transfect millions of maize protoplasts for testing large libraries of plasmids in maize mesophyll cells.

Research

JoVE Journal - Biology
Free Sample

Rapid Analysis of Circadian Phenotypes in Arabidopsis Protoplasts Transfected with a Luminescent Clock Reporter

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Cited by 18 •

2016

The circadian clock regulates about a third of the Arabidopsis transcriptome, but the percentage of genes that feed back into timekeeping remains unknown. Here we visualize a method to rapidly assess circadian phenotypes in any mutant line of Arabidopsis using luminescent imaging of a circadian reporter transiently expressed in protoplasts.

Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window

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Cited by 12 •

2012

By combining a polished and reinforced thin-skull (PoRTS) cranial window and glioblastoma (GBM) cell injection, we can observe glioma initiation and growth from injected GBM cells in the brain of a live mouse longitudinally.

Acute Brain Trauma in Mice Followed By Longitudinal Two-photon Imaging

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Cited by 12 •

2014

Acute brain trauma is a severe injury that has no adequate treatment to date. Multiphoton microscopy allows studying longitudinally the process of acute brain trauma development and probing therapeutical strategies in rodents. Two models of acute brain trauma studied with in vivo two-photon imaging of brain are demonstrated in this protocol.

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