By combining a polished and reinforced thin-skull (PoRTS) cranial window and glioblastoma (GBM) cell injection, we can observe glioma initiation and growth from injected GBM cells in the brain of a live mouse longitudinally.
Zhang, L., Lapierre, A., Roy, B., Lim, M., Zhu, J., Wang, W., Sampson, S. B., Yun, K., Lyons, B., Li, Y., Lin, D. Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window. J. Vis. Exp. (69), e4201, doi:10.3791/4201 (2012).