Spread Plate Method
Spread Plate Method - Related Videos
18:00
Aseptic Laboratory Techniques: Plating Methods
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Cited by 438 •
2012
When working with media and reagents used to culture microorganisms, aseptic technique must be practiced to ensure contamination is minimized. A variety of plating methods are routinely used to isolate, propagate, or enumerate bacteria and phage, all of which incorporate procedures that maintain the sterility of experimental materials.
02:25
Spreading of Chromatin Modifications
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2020
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex. Writers The writer is an enzyme that can...
12:06
Surface Spreading and Immunostaining of Yeast Chromosomes
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Cited by 36 •
2015
A method for surface-spreading chromosomes from budding yeast is presented. This method is derived from a method previously described by Loidl and Klein. In addition, we demonstrate a procedure for immunostaining of spread chromosomes.
06:38
Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes
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Cited by 27 •
2017
Meiosis is the developmental process by which gametes are formed through a single round of DNA replication and two successive rounds of chromosome segregation. Mammalian meiosis can be examined by utilizing a technique to prepare meiotic chromosome spreads. Here, we demonstrate a method of preparing surface-spread nuclei from mouse spermatocytes.
11:31
Determination of Impingement Forces on a Flat Plate with the Control Volume Method
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2023
Source: Ricardo Mejia-Alvarez and Hussam Hikmat Jabbar, Department of Mechanical Engineering, Michigan State University, East Lansing, MI The purpose of this experiment is to demonstrate forces on bodies as the result of changes in the linear momentum of the flow around them using a control volume formulation [1, 2]. The control volume analysis focuses on the macroscopic effect of flow on engineering systems, rather than the detailed description that could be achieved with a differential...
FAQs
Serial dilution reduces the number of microorganisms delivered in the measured aliquot, making growth on the agar surface suitable for observing separate colonies and estimating concentration. The resulting colony count can be related to the diluted sample to assess the original microbial population in the tested sample.
A colony-forming unit, or CFU, is the measurement used to estimate viable microbial concentration from the colonies that appear after incubation. A single visible colony may arise from one viable cell or from a cell cluster, so CFU counts describe colony-producing units rather than necessarily the exact number of individual cells. This distinction matters when comparing populations.
A sterile glass or plastic spreader distributes the aliquot across the agar surface. Even distribution helps place microorganisms in separate locations, supporting the development of isolated colonies that can be counted or selected. Sterility is also important because the spreader should not introduce additional microorganisms that could alter the observed population.
The workflow begins with serial dilution of the liquid sample. A measured aliquot is then placed on solid growth medium, spread evenly across the agar with a sterile glass or plastic spreader, and incubated. After growth occurs, researchers count the visible colonies, use CFUs to estimate concentration, and may use isolated colonies for identification or further experimentation.
The essential components are a liquid sample, serial dilution steps, solid growth medium in an agar plate, a measured aliquot, and a sterile glass or plastic spreader. Incubation follows surface spreading and allows viable microorganisms to produce visible colonies. Together, these materials and conditions connect the starting sample to an observable colony count.
It is useful when researchers need both an estimate of viable microbial concentration and separated colonies for later study. Applications described for the method include bacterial culture, environmental microbiology, food and water testing, and experiments that track population changes. The resulting colonies can support identification or further experimentation when isolated growth is required.