Sterile Filtration
Sterile Filtration - Related Videos
01:32
Filtration and Urine Formation
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2019
The function of the kidneys is to filter, reabsorb, secrete, and excrete. Every day the kidneys filter nearly 180 liters of blood, initially removing water and solutes but ultimately returning nearly all filtrates into circulation with the help of osmoregulatory hormones. This process removes wastes and toxins but is also crucial to maintain water and electrolyte levels. Most of these functions are performed by the tiny but numerous nephrons contained within the kidneys. Blood enters the renal...
01:26
Glomerular Filtration: Net Filtration Pressure
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2025
Glomerular filtration, a key process in the kidneys, is regulated by three main pressures: Glomerular blood hydrostatic pressure (GBHP), Capsular hydrostatic pressure (CHP), and Blood colloid osmotic pressure (BCOP). GBHP, with an average value of 55 mmHg, promotes filtration by pushing water and solutes through the filtration membrane. This is balanced by two opposing forces: CHP, a "back pressure" exerted against the filtration membrane by fluid already in the capsular space and renal tubule,...
12:29
Sterile Tissue Harvest
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN In 1959 The 3 R's were introduced by W.M.S. Russell and R.L. Burch in their book The Principles of Humane Experimental Technique. The 3 R's are replacement, reduction, and refinement of the use of animals in research. The use of cell lines and tissue cultures that originated from research animals is a replacement technique, as it allows for many experiments to be conducted in vitro. Harvesting...
00:53
Filtration
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2024
Filtration is a physical separation process that involves passing a suspension through a porous medium to separate solids from fluids. During filtration, solids collect on the porous medium while liquids, also collectively known as the filtrate, pass through. The filtration medium is selected based on the filtration purpose, quantity, and nature of the precipitate. The general criteria for a suitable filtering medium are that it is inert, mechanically strong, nonabsorbent toward dissolved...
10:58
Isolation of Fecal Bacteria from Water Samples by Filtration
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Luisa Ikner The quality of water destined for use in agricultural, recreational, and domestic settings is of great importance due to the potential for outbreaks of waterborne disease. Microbial agents implicated in such events include parasites, bacteria, and viruses that are shed in high numbers in the feces of infected people and animals. Transmission to new and susceptible hosts may...
FAQs
Pore size determines which microorganisms and particulate contaminants the membrane can retain, while membrane properties influence how the fluid or gas passes through it. Operating conditions also affect performance, including the balance between contaminant removal and flow. Selecting these factors together helps researchers obtain a filtered material suitable for contamination-sensitive biological work.
Sterile filtration removes contaminants without exposing the material to heat. This makes the approach useful when researchers need to protect biological samples while maintaining aseptic conditions. The heat-free mechanism supports preparation of materials used in biology, including solutions and culture-related supplies, where preserving the sample during contamination control is important.
The same physical separation principle can be applied to gases as well as liquids. Air passes through a membrane that retains microorganisms and particulate contaminants, helping provide an aseptic air supply. This is relevant to biological systems in which airborne contamination could compromise samples, cultures, or other contamination-sensitive experimental conditions.
A basic workflow begins by selecting a membrane with properties and pore size appropriate for the fluid or gas and the contaminants that must be retained. The material is then passed through the membrane under suitable operating conditions, and the filtered output is collected for biological use. Performance depends on the selected membrane and filtration conditions.
Researchers apply Sterile Filtration to culture media, buffers, pharmaceutical solutions, and air supplies. These materials may support cell culture, microbiology, and other experiments in which contamination can interfere with biological results. The method is especially relevant when researchers need to prepare a material while maintaining aseptic conditions without using heat.
Successful filtration produces a liquid or gas in which microorganisms and particulate contaminants have been retained by the membrane. In cell culture and microbiology, this helps prepare media, buffers, or air supplies before they contact contamination-sensitive materials. The quality of the outcome depends on pore size, membrane properties, and operating conditions.