Steroids
Steroids - Related Videos
11:07
Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
0 Views •
Cited by 1 •
2011
An in vitro method for preparing functional glucocorticoid receptor (GR)•hsp90 protein complexes from purified proteins and cellular lysates is described. The method utilizes immunoadsorption of recombinant GR followed by salt-stripping and protein complex reconstitution. The importance of cofactors and buffer conditions are discussed, as are potential method applications.
05:55
Tension-Free Weight-Bearing Model of Steroid-Induced Osteonecrosis of Femoral Head in Rats
0 Views •
2024
The protocol shows building a simple and cost-effective rat weight-bearing training model for steroid-induced osteonecrosis of the femoral head using elastic therapeutic tape.
08:39
High-throughput Image Analysis of Tumor Spheroids: A User-friendly Software Application to Measure the Size of Spheroids Automatically and Accurately
0 Views •
Cited by 117 •
2014
We present a high-throughput image analysis software application to measure the size of three-dimensional tumor spheroids imaged with bright-field microscopy. This application provides a fast and effective way to examine the effects of therapeutic drugs on spheroids, which is beneficial for researchers who wish to use spheroids in drug screens.
12:19
Three-Dimensional (3D) Tumor Spheroid Invasion Assay
0 Views •
Cited by 222 •
2015
Invasion of surrounding normal tissues is a defining characteristic of malignant tumors. We provide here a simple, semi-automated micro-plate assay of invasion into a natural 3D biomatrix that has been exemplified with a number of models of advanced human cancers.
12:04
Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
0 Views •
Cited by 39 •
2012
We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.
FAQs
Lipid solubility allows many steroid hormones to pass through cell membranes, giving them access to intracellular receptors. Other steroid-responsive pathways involve membrane-associated receptors, which can produce rapid signaling effects. Receptor location therefore helps determine whether a response is expressed mainly through altered gene expression or through a faster signaling route.
Cholesterol has two distinct biological roles: it helps regulate membrane fluidity and provides a precursor for steroid hormone production. These functions connect membrane organization with endocrine signaling. Changes affecting cholesterol can therefore be relevant to both the physical properties of cellular membranes and the availability of molecules used to produce steroid hormones.
Steroid signaling can produce different temporal and cellular outcomes depending on the receptor pathway involved. Intracellular receptor interactions can alter gene expression, changing how the cell regulates selected functions. Membrane-associated receptor interactions can instead support rapid signaling. This distinction helps researchers relate receptor location to the speed and character of a biological response.
A complete investigation can follow steroids across synthesis, transport, receptor interaction, and breakdown. Examining these stages helps connect production with movement through biological systems, cellular response, and eventual inactivation or removal. This framework is useful for identifying where signaling may be regulated and for interpreting steroid effects in physiology or disease mechanisms.
Steroid research supports endocrinology, pharmacology, development, and disease-mechanism studies. Investigators can examine how steroid synthesis, transport, receptor binding, or breakdown relates to physiological outcomes. These approaches help connect molecular signaling with broader processes such as metabolism, stress responses, reproduction, and development without treating any single pathway as isolated from the others.
Corticosteroids and sex steroids provide distinct contexts for examining steroid action. Corticosteroid research can address metabolism and stress responses, whereas sex steroid research can focus on reproduction and development. Comparing these groups helps researchers investigate how related steroid-based signaling systems influence different physiological processes while sharing broader principles of receptor-mediated regulation.