Three-Dimensional (3D) Tumor Spheroid Invasion Assay

Journal

Medicine

JoVE Journal
Medicine

This content is Free Access.

JoVE Journal Medicine

Three-Dimensional (3D) Tumor Spheroid Invasion Assay

DOI:

10.3791/52686-v

•

12:19 min

•

May 01, 2015

•

Maria Vinci², Carol Box, Suzanne A. Eccles

¹Division of Molecular Pathology,The Institute of Cancer Research, ²Division of Cancer Therapeutics,The Institute of Cancer Research

Chapters

00:05Title
02:323D Tumor Spheroid Invasion Assay
04:57Automated Image Acquisition and Analysis
07:21Manual Image Acquisition and Analysis
09:36Results: Exemplification of Different Types of Cancer Cell Invasion
11:22Conclusion

Summary

Automatic Translation

Invasion of surrounding normal tissues is a defining characteristic of malignant tumors. We provide here a simple, semi-automated micro-plate assay of invasion into a natural 3D biomatrix that has been exemplified with a number of models of advanced human cancers.

Three-Dimensional (3D) Tumor Spheroid Invasion Assay

•

•

•

Chapters

Summary

Automatic Translation

Tags

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Spheroid Assay to Measure TGF-β-induced Invasion

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

Three-dimensional Imaging of Nociceptive Intraepidermal Nerve Fibers in Human Skin Biopsies

Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

Quantification of Breast Cancer Cell Invasiveness Using a Three-dimensional (3D) Model

Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time

Three-dimensional Co-culture Model for Tumor-stromal Interaction

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells

Three-Dimensional Culture Assay to Explore Cancer Cell Invasiveness and Satellite Tumor Formation

Read Article