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JoVE Journal

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Three-Dimensional (3D) Tumor Spheroid Invasion Assay

Three-Dimensional (3D) Tumor Spheroid Invasion Assay

Article DOI: 10.3791/52686 12:19 min
May 1st, 2015


Summary May 1st, 2015

Invasion of surrounding normal tissues is a defining characteristic of malignant tumors. We provide here a simple, semi-automated micro-plate assay of invasion into a natural 3D biomatrix that has been exemplified with a number of models of advanced human cancers.

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