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Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy
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JoVE Journal Medicine
Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

Human Neuroendocrine Tumor Cell Lines as a Three-Dimensional Model for the Study of Human Neuroendocrine Tumor Therapy

DOI:
10.3791/4218-v

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12:04 min

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August 14, 2012

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, , , ,
1Raymond and Beverly Sackler Foundation, 2The Cancer Institute of New Jersey,University of Medicine and Dentistry of New Jersey, 3School of Natural Sciences,Institute for Advanced Study, Princeton, New Jersey

Chapters

  • 00:05Title
  • 02:05Preparation of 1% Agarose-coated 24-well Plates
  • 03:08Preparation of Single Cell Suspensions
  • 04:17Spheroid Culture
  • 05:31Drug Treatment and Monitoring of 3D Spheroid Cultures
  • 07:22HistoGel-embedding of the NET 3D Spheroids
  • 09:06Results: Culture of NET 3D Spheroids and Their Response to Drug Treatment
  • 11:29Conclusion

Summary

Automatic Translation

Automatic Translation

We present a simple agarose overlay platform to grow 3D multicellular spheroids using neuroendocrine cancer cell lines. This method provides a very convenient way to examine the effect of therapeutic drugs on the neuroendocrine tumor cells. It could also help us establish human neuroendocrine tumor spheroids for cancer therapy.

Tags

Neuroendocrine TumorHuman Cell LinesThree-dimensional ModelStudyTherapyIncidenceMalignanciesSystemic TherapyBiologyTherapeutic TargetsNonresectableMetastatic Neuroendocrine Tumors3D Cell CultureDrug ScreeningMicroenvironmentMulticellular SpheroidsIn Vitro ModelAgarose-coated PlatePhysiological ConditionsDrug Treatments

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