Su-8 Master Mold

An SU-8 master mold is a patterned relief structure used to replicate microscale features in materials such as polydimethylsiloxane (PDMS), supporting the fabrication of devices for biological research. To create it, a thick SU-8 epoxy photoresist is spin-coated onto a substrate, selectively exposed to ultraviolet light through a photomask, and post-exposure baked to crosslink the illuminated regions; development removes the uncrosslinked resist. The resulting mold defines channels, chambers, or other microstructures when liquid PDMS is cast and cured against it. These molds enable reproducible microfluidic platforms for cell culture, tissue engineering, diagnostics, and studies of cellular behavior under controlled conditions.

Su-8 Master Mold - Related Videos

Research

JoVE Journal - Engineering

Fabrication of a Master Mold for Microneedles with a Micron-sized Air-vent Hole

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2025

This article introduces a unique method for fabricating master molds with an air-vent hole to prevent air entrapment during viscous polymer casting, enabling the fabrication of gelatin microneedles with sharp tips. By reducing the casting volume of high-viscosity gelatin, thin-walled hollow pyramidal microneedles could also be fabricated.

Custom Engineered Tissue Culture Molds from Laser-etched Masters

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Cited by 8 •

2018

Herein we present a rapid, facile, and low-cost method for fabricating custom polydimethylsiloxane molds that can be used for producing hydrogel-based engineered tissues with complex geometries. We additionally describe results from mechanical and histological assessments conducted on engineered cardiac tissues produced using this technique.

Education

JoVE Core - Molecular Biology

Master Transcription Regulators

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2020

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Master Transcription Regulators

0 Views •

2023

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Rapid and Low-cost Prototyping of Medical Devices Using 3D Printed Molds for Liquid Injection Molding

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Cited by 34 •

2014

We have devised a method for low-cost and rapid prototyping of liquid elastomer rubber injection molded devices by using fused deposition modeling 3D printers for mold design and a modified desiccator as a liquid injection system.

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