Transgenic Plant Regeneration
Transgenic Plant Regeneration - Related Videos
02:50
Transgenic Plants
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2021
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms. The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
05:09
Binary Bacterial Artificial Chromosome Vector Based Gene Transformation in Arabidopsis Plants: A Technique to Generate Transgenic Plants With Single-Copy Insertion
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2025
In this video, we demonstrate generation of transgenic Arabidopsis plants expressing stable transgene through Agrobacterium-mediated binary bacterial artificial chromosome-based gene transformation.
13:37
Generation of Composite Plants in Medicago truncatula used for Nodulation Assays
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Cited by 7 •
2011
We demonstrate how hairy root composite plants can be used to study plant-rhizobium interactions and nodulation in the difficult-to-transform species Medicago truncatula.
06:30
Efficient Regeneration-based Agrobacterium-Mediated Transformation of an Asexual Amphibious Brassicaceae Species, Rorippa aquatica
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2026
We present an efficient, optimized protocol for Agrobacterium-mediated transformation of an asexual amphibious Brassicaceae species, Rorippa aquatica, without resource-intensive sterile tissue culture procedures. Utilizing a visual transformation marker and shoot regeneration pipeline successfully generated near clonal transformants, thereby advancing the future studies of plant's adaptation to the underwater environment.
12:08
Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
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Cited by 4 •
2018
Using a pBIBAC-GW binary vector makes generating transgenic plants with intact single-copy insertions, an easy process. Here, a series of protocols is presented that guide the reader through the process of generating transgenic Arabidopsis plants, and testing the plants for intactness and copy number of the inserts.
FAQs
Plant growth regulators guide transformed cells or tissues toward developmental responses needed to produce a plant. Under controlled culture conditions, they can stimulate callus formation, organogenesis, or somatic embryogenesis. These responses provide different routes from modified biological material to regenerated plants, making regulator-controlled culture a central part of recovering plants that retain the introduced genetic material.
Selectable conditions help identify cells that retain the introduced transgene among the cultured material. This narrows subsequent regeneration efforts toward transformed cells rather than treating every cultured cell as genetically modified. Selection therefore supports the transition from transformation to plant recovery, although regenerated plants still require later evaluation for stable gene integration and expression.
Stable gene integration and gene expression provide complementary information about the introduced DNA. Integration testing asks whether the transgene remains stably incorporated, while expression testing examines whether it is active in the regenerated plant. Evaluating both outcomes helps distinguish plants that merely carry the genetic material from those suitable for gene-function studies or trait development.
The workflow begins with transformation of plant cells or tissues, followed by culture under controlled conditions and selection for material retaining the transgene. Regeneration then proceeds through an appropriate culture response, such as callus formation, organogenesis, or somatic embryogenesis. Scientists acclimatize and propagate the resulting plants before evaluating integration and expression.
Acclimatization is a required transition between plant regeneration under controlled culture conditions and continued growth outside that initial setting. Once plants are acclimatized, they can be propagated and examined as developing whole plants rather than only as cultured material. This stage supports recovery of plants that can be evaluated for stable genetic characteristics and expression.
Transgenic plant regeneration supports functional genomics by enabling researchers to examine gene function in whole plants. It also contributes to crop improvement and stress-tolerance research, while allowing development of plants with valuable agricultural or pharmaceutical traits. Because the regenerated plants can be propagated and evaluated, the approach connects genetic modification with observable plant-level outcomes.