Tyrosine Recombinase
Tyrosine Recombinase - Related Videos
12:02
Molecular Evolution of the Tre Recombinase
0 Views •
Cited by 4 •
2008
Here we report the generation of Tre recombinase through directed, molecular evolution. Tre recombinase recognizes a pre-defined target sequence within the LTR sequences of the HIV-1 provirus, resulting in the excision and eradication of the provirus from infected human cells. While still in its infancy, directed molecular evolution will allow the creation of custom enzymes that will serve as tools of molecular surgery and molecular medicine.
07:06
Principles of Site-Specific Recombinase (SSR) Technology
0 Views •
Cited by 14 •
2008
The advent of site-specific recombinase (SSR) technology and the Cre/lox system has led to numerous advances in molecular biology, and has proven itself as a valuable tool for assessing gene function in transgenic animals. This interview discusses the mechanism of site specific recombination by Cyclization recombinase (Cre) and how the use of this enzyme has led to the development of conditional mutagenesis, which has significant advantages over traditional knock out strategies.
01:26
Receptor Tyrosine Kinases
0 Views •
2025
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
10:20
Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase
0 Views •
Cited by 2 •
2008
Current HIV-1 strategies act to suppress the viral life cycle but do not effectively eradicate infection. Here, we demonstrate that an engineered recombinase can efficiently excise integrated HIV-1 proviral DNA from the genome of infected cells.
10:49
Pre-clinical Evaluation of Tyrosine Kinase Inhibitors for Treatment of Acute Leukemia
0 Views •
Cited by 6 •
2013
Receptor tyrosine kinases are ectopically expressed in many cancers and have been identified as therapeutic targets in acute leukemia. This manuscript describes an efficient strategy for pre-clinical evaluation of tyrosine kinase inhibitors for the treatment of acute leukemia.
FAQs
The active-site tyrosine provides the chemical linkage that temporarily connects the enzyme to cleaved DNA. After one strand is cut, this covalent DNA-enzyme intermediate preserves the reaction state while the partner strand undergoes exchange. Re-ligation then releases the DNA in a rearranged configuration, allowing strand transfer to proceed without permanently leaving the cleavage site open.
Recognition-site orientation determines which architectural outcome follows. If the sites are arranged in one configuration, a segment can be integrated or excised; another orientation can invert the intervening DNA, while a resolution reaction separates linked products. Thus, the same catalytic strategy can produce different genome changes because the geometry of the target sites changes.
Unlike rearrangement mechanisms that depend on extensive matching sequence, tyrosine recombinases act at defined recognition sites. Their specificity therefore comes from identifying and pairing those target sequences rather than from broad homology across the DNA segment. This makes the reaction suitable for controlled changes at selected locations, including engineered genome manipulations.
In engineered biology, Cre-lox exemplifies how this chemistry can be converted into a programmable genome tool. Researchers can use it for conditional gene deletion, lineage tracing, transgenesis, and controlled genome engineering. These applications rely on defined recognition sites positioned around or within genetic elements so recombination changes the selected DNA arrangement rather than requiring extensive homology.
Tyrosine recombinases can regulate microbial genomes and participate in bacteriophage life cycles by changing DNA arrangements at specific sites. Their possible outputs, including integration, excision, inversion, and resolution, give biological systems several ways to alter genome organization. Their activity therefore links a precise molecular reaction to changes in genome state and phage life cycles.
Analysis should follow the reaction in order: identify the two recognition sites, assess their orientation, track strand cleavage and the temporary DNA-enzyme intermediate, and determine whether re-ligation yields integration, excision, inversion, or resolution. This sequence connects the starting DNA design to the expected rearrangement and helps interpret the resulting genetic configuration.