Upright Mounting
Upright Mounting - Related Videos
07:34
Upright Imaging of Drosophila Embryos
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Cited by 7 •
2010
Here we present a mounting protocol for stained Drosophila embryos in an upright position that allows imaging of cross-sections using Confocal microscopy.
12:29
Upright Imaging of Drosophila Egg Chambers
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Cited by 1 •
2015
The upright imaging method described in this protocol allows for the detailed visualization of the poles of a developing Drosophila melanogaster egg. This end-on view provides a new perspective into the arrangements and morphologies of multiple cell types in the follicular epithelium.
03:34
Agar Mounting: A Basic Method of Mounting Live Zebrafish Embryos for Long-Term Imaging
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2023
This video describes step by step instructions on mounting live zebrafish embryos on 6 well plate using low melt agarose. It also provides the process to acquire images of live embryos and analyze it using software.
09:47
Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis
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Cited by 13 •
2015
The ex vivo upright droplet culture is an alternative to current in vitro and in vivo experimental techniques. This protocol is easy to perform and requires smaller amounts of reagent, while permitting the ability to manipulate and study fetal vascularization, morphogenesis, and organogenesis.
08:13
Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
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Cited by 9 •
2018
We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.
FAQs
Maintaining a vertical or anatomically intended orientation preserves the relative positions of visible structures. This makes spatial relationships easier to interpret because features remain aligned with the specimen’s expected organization rather than being displaced by preparation. The resulting images can support clearer anatomical assessment and more reliable comparisons among specimens or observations.
Some tissues, embryos, organisms, and other structured specimens have forms or behaviors that depend on their orientation. Changing that orientation can make anatomical relationships or observable behavior harder to interpret. Upright mounting keeps the specimen aligned with its relevant biological arrangement, helping microscopy reveal structure in a context that remains meaningful for analysis.
A mounting medium or physical restraint stabilizes the specimen while its intended orientation is maintained. This support helps prevent unwanted movement or positional change during preparation and observation, which can otherwise complicate image interpretation. The selected support therefore contributes to preserving the specimen’s arrangement and obtaining consistent microscopy results.
Upright mounting deliberately preserves a vertical or anatomically intended arrangement, whereas an arbitrary orientation may make the specimen’s normal spatial organization less apparent. The distinction matters when researchers need to interpret anatomical relationships, compare images, or document form consistently. Orientation-aware preparation reduces ambiguity caused by viewing structured material from an unintended position.
Preparation begins by identifying the specimen’s vertical or anatomically intended orientation. The sample is then positioned on a slide, holder, or comparable support and stabilized with a suitable mounting medium or restraint. That orientation must remain controlled through preparation and microscopy, after which images can be collected and documented for interpretation or comparison.
The support should keep the specimen stable while preserving the orientation needed for observation. Depending on the sample and preparation, this may involve a slide, holder, or another support used with an appropriate mounting medium or restraint. The practical goal is not simply to hold the sample, but to prevent orientation changes that could affect interpretation.
This approach is particularly relevant for tissues, embryos, organisms, and other structured specimens whose form or behavior depends on orientation. Keeping these samples aligned can clarify anatomical organization and preserve relationships that are important during microscopy. It is therefore useful when researchers need to examine structured biological material rather than view isolated features without spatial context.
By reducing distortion and preserving anatomical organization, upright mounting can improve image interpretation and support consistent imaging. Those consistent views are useful for comparative analysis across specimens or preparations and for documenting observations. The technique therefore contributes not only to individual microscopy observations, but also to organized records and more comparable biological assessments.